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[Observation of pollen tube germination of Platycodon grandiflorum by fluorescence microscopy]
Jian-he Wei1, Feng-jie Ma, Lu-qi Huang
1Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Chinese Peking Union Medical College, Beijing 100094, China.
Objective:
To determine the self-compatibility of Platycodon grandiflorum and the location of microspore germination on stigma.
Method:
The microspore germination of self-pollination, self-plant-pollination and cross-pollination and the pollination microspore germination in and outside of stigma were observed with fluorescence microscopy.
Result:
Most pollens of self-pollination, self-plant-pollination and cross-pollination can germinate on the stigma, and after 24 hours, pollen tube entered into the ovary successfully. Pollinated on the outer-surface of stigma, microspores could not germinate, but on the inner-surface of the stigma when it dehisced most microspores can germinate.
Conclusion:
The compatibility of self-plant-pollination of Platycodon grandiflorum is high. The microspore germination loci is on the inner-surface of the dehisced stigma.
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