Related Experiment Video
Updated: Jul 10, 2026

An Efficient Clearing Protocol for the Study of Seed Development in Tomato (Solanum lycopersicum L.)
Published on: September 7, 2022
Changes in tomato ovary transcriptome demonstrate complex hormonal regulation of fruit set
Wim H Vriezen1, Richard Feron1, Fabio Maretto1
1Department of Plant Cell Biology, Radboud University Nijmegen, Toernooiveld 1, 6525ED Nijmegen, the Netherlands.
Plant hormones like ethylene and abscisic acid (ABA) regulate tomato fruit set. Their expression decreases after pollination, suggesting an antagonistic role to auxin and gibberellin in preventing premature fruit development.
Area of Science:
- Plant biology
- Molecular biology
- Hormone signaling
Background:
- Plant hormones mediate fruit development post-pollination.
- Phytohormones play a crucial role in initiating fruit set.
Purpose of the Study:
- Investigate the role of phytohormones in tomato (Solanum lycopersicum) fruit set.
- Analyze gene expression profiles in ovaries to understand hormone regulation.
Main Methods:
- Employed two complementary transcriptome analysis approaches: cDNA-amplified fragment length polymorphism (AFLP) and microarray analysis.
- Examined gene expression patterns in tomato ovaries.
Main Results:
- Gene expression profiles indicate involvement of auxin, gibberellin, ethylene, and abscisic acid (ABA) in fruit set.
- High expression of ethylene and ABA biosynthesis genes observed in ovaries before fruit development.
- Pollination or gibberellin application attenuated ethylene and ABA gene expression within 24 hours.
Conclusions:
- Ethylene and ABA may act antagonistically to auxin and gibberellin in fruit set.
- ABA and ethylene likely maintain the ovary in a dormant state, preventing premature fruit development.
- This hormonal balance protects ovary tissue and ensures development occurs after pollination and fertilization.
More Related Videos
09:33An Efficient Method for Quantitative, Single-cell Analysis of Chromatin Modification and Nuclear Architecture in Whole-mount Ovules in Arabidopsis
Published on: June 19, 2014
15:25Tomato Analyzer: A Useful Software Application to Collect Accurate and Detailed Morphological and Colorimetric Data from Two-dimensional Objects
Published on: March 16, 2010
Related Concept Videos
Hormonal Control of the Ovarian Cycle
Before puberty, the hypothalamus releases GnRH in a low frequency, low amplitude pulsatile manner. This along with the immature hypothalamic-pituitary-gonadal axis activity, results in low estrogen levels and the absence of a fully functional ovarian cycle. At puberty, GnRH secretion increases in both frequency and...
Hormonal Regulation of the Menstrual Cycle
At puberty, GnRH begins a pulsatile release pattern, which triggers the anterior pituitary gland to secrete follicle-stimulating hormone (FSH) and luteinizing hormone (LH). The frequency and amplitude of GnRH pulses vary across the menstrual cycle, with faster pulses favoring LH release and slower pulses favoring FSH release.
Hormonal Regulation
Hormonal Regulation
Gene Regulation During Sporulation
Fruit Development, Structure, and Function