Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Highly selective amperometric glucose microdevice derived from diffusion layer gap electrode.

Wen-Zhi Jia1, Yu-Lin Hu, Yan-Yan Song

  • 1Key Lab of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China.

Biosensors & Bioelectronics
|November 22, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Synthesis of a hydrophilic poly-l-lysine/graphene hybrid through multiple non-covalent interactions for biosensors.

Journal of materials chemistry. B·2020
Same author

Multiscale evaluation of pore curvature effects on protein structure in nanopores.

Journal of materials chemistry. B·2020
Same author

Rationally designed two-photon absorption compounds based on benzoxazole derivatives: structure-property relationships and bio-imaging applications.

Journal of materials chemistry. B·2020
Same author

A meta-analysis of the vascular endothelial growth factor polymorphisms associated with the risk of pre-eclampsia.

Bioscience reports·2020
Same author

Super-kdr mutation M918L and multiple cytochrome P450s associated with the resistance of Rhopalosiphum padi to pyrethroid.

Pest management science·2020
Same author

Rapid thermal deposited GeSe nanowires as a promising anode material for lithium-ion and sodium-ion batteries.

Journal of colloid and interface science·2020
Same journal

Integration of electrochemical sensors in organ-on-a-chip microfluidic platforms: Advances and perspectives.

Biosensors & bioelectronics·2026
Same journal

DNN-PURE: A deep neural network approach to paper-based urea sensing.

Biosensors & bioelectronics·2026
Same journal

Rationally architected MOF-derived Co<sub>3</sub>O<sub>4</sub>@NiMn-LDH hollow heterostructure-based sensor array empowering sensitive detection and discrimination of neurological biomarkers.

Biosensors & bioelectronics·2026
Same journal

Four-in-one multifunctional CoCu-NC@AuPt nanozyme integrated M13 phage-displayed nanobody based multimodal lateral flow immunoassay for bovine lactoferrin detection.

Biosensors & bioelectronics·2026
Same journal

A novel capillary-driven dual-mode imaging flow cytometry system for malaria parasite detection and quantification.

Biosensors & bioelectronics·2026
Same journal

Liver-targeted alkaline phosphatase-activatable fluorescent probe for imaging liver fibrosis and screening anti-fibrotic natural products.

Biosensors & bioelectronics·2026
See all related articles

This study presents a novel glucose biosensor that overcomes nonselective detection issues. The diffusion layer gap electrode pair design enables highly selective amperometric glucose sensing, even in the presence of interfering substances.

Area of Science:

  • Electrochemistry
  • Biosensor Technology
  • Analytical Chemistry

Background:

  • Enzyme-based biosensors often suffer from nonselective detection of reaction products.
  • Improving selectivity is crucial for reliable electrochemical biosensor fabrication.
  • Existing amperometric detection methods lack specificity for enzymatic reaction products.

Purpose of the Study:

  • To design a highly selective amperometric glucose biosensor.
  • To address the challenge of nonselective detection in enzyme-based electrochemical biosensors.
  • To utilize a diffusion layer gap electrode pair for enhanced selectivity.

Main Methods:

  • Fabrication of a gold tube electrode coated with glucose oxidase/Nafion/graphite.
  • Implementation of a diffusion layer gap electrode pair with a Pt probe electrode.

Related Experiment Videos

  • Electrochemical oxidation of interfering species within the diffusion layer.
  • Systematic investigation of parameters influencing interference-removing efficiency.

    • Main Results:

    • Achieved interference-free glucose detection with optimized parameters (30s electrolyzing time, 3mm tip-tube distance, 0.4V tube electrode potential).
    • Demonstrated linear electrochemical response to glucose concentration (1 x 10^-5 to 4 x 10^-3 M) with high correlation coefficients (0.9936 without interferents, 0.9995 with interferents).
    • Validated effectiveness through numerical simulation, showing good agreement with experimental data.

    Conclusions:

    • The developed diffusion layer gap electrode pair biosensor offers high selectivity for amperometric glucose detection.
    • This approach effectively removes interfering electroactive species, enhancing sensor reliability.
    • The findings provide a promising strategy for fabricating selective enzyme-based electrochemical biosensors.