Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Complexometric Titration: Ligands00:43

Complexometric Titration: Ligands

Different monodentate and polydentate ligands are used as complexing agents in complexometric titration reactions. The formation of complexes by mono- and bidentate ligands involves two or more intermediate steps, limiting their use as complexing agents. In comparison, polydentate ligands can form complexes with metal ions in a single-step process, facilitating sharper end points. This means polydentate ligands, such as amino carboxylic acid derivatives, are most commonly employed in...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Methylation of DNA duplexes regulates cGAS-mediated innate immune activation <i>via</i> condensate formation.

RSC chemical biology·2026
Same author

5-Formylcytosine functions as a chemical regulator of nucleosome positioning.

Nature communications·2026
Same author

Disruption of afferent neural circuits leads to arrhythmia in the animal model of hereditary sensory and autonomic neuropathy 6.

Frontiers in neural circuits·2026
Same author

Earliest octopuses were giant top predators in Cretaceous oceans.

Science (New York, N.Y.)·2026
Same author

Artificial intelligence-driven whole-brain cell mapping with highly multiplexed in situ hybridization.

Neuron·2026
Same author

Expansive spatial pattern of Aβ deposition in patients with cerebral amyloid angiopathy: A three-dimensional surface-to-depth analysis.

Science advances·2026

Related Experiment Video

Updated: Jul 10, 2026

DNA Methylation: Bisulphite Modification and Analysis
12:34

DNA Methylation: Bisulphite Modification and Analysis

Published on: October 21, 2011

DNA methylation analysis using metal complex formation.

Akimitsu Okamoto1, Kazuo Tanaka, Kazuki Tainaka

  • 1Frontier Research System, RIKEN, Wako, Saitama 351-0198, Japan. aki-okamoto@riken.jp

Nucleic Acids Symposium Series (2004)
|November 22, 2007
PubMed
Summary
This summary is machine-generated.

Researchers developed a new chemical method for detecting 5-methylcytosine (M) in DNA. This selective oxidation process efficiently distinguishes M from cytosine (C), aiding genetic analysis.

More Related Videos

Methodology for Accurate Detection of Mitochondrial DNA Methylation
12:11

Methodology for Accurate Detection of Mitochondrial DNA Methylation

Published on: May 20, 2018

Methylated DNA Immunoprecipitation
21:24

Methylated DNA Immunoprecipitation

Published on: January 2, 2009

Related Experiment Videos

Last Updated: Jul 10, 2026

DNA Methylation: Bisulphite Modification and Analysis
12:34

DNA Methylation: Bisulphite Modification and Analysis

Published on: October 21, 2011

Methodology for Accurate Detection of Mitochondrial DNA Methylation
12:11

Methodology for Accurate Detection of Mitochondrial DNA Methylation

Published on: May 20, 2018

Methylated DNA Immunoprecipitation
21:24

Methylated DNA Immunoprecipitation

Published on: January 2, 2009

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Chemical Biology

Background:

  • Analyzing gene expression requires understanding cytosine methylation status.
  • Distinguishing 5-methylcytosine (M) from cytosine (C) in DNA is challenging due to the subtle difference of a single methyl group.
  • Efficient methods for site-specific methylation analysis are crucial for genetic research.

Purpose of the Study:

  • To develop a rapid and selective chemical reaction for distinguishing 5-methylcytosine (M) from cytosine (C).
  • To enable efficient analysis of cytosine methylation status at specific gene sites.

Main Methods:

  • Utilizing M-selective oxidation with an osmium complex.
  • Employing functional bipyridine ligands to enhance selectivity.
  • Observing differential oxidation rates between M and C.

Main Results:

  • 5-methylcytosine (M) was efficiently oxidized by the osmium complex reaction mixture.
  • Cytosine (C) exhibited very weak oxidation under the same conditions, allowing clear distinction.
  • Information on methylation status at specific DNA sites was readily obtained.

Conclusions:

  • M-selective oxidation provides a powerful tool for analyzing DNA methylation.
  • This method facilitates the distinction between 5-methylcytosine and cytosine, crucial for genetic studies.
  • The use of functional bipyridine ligands enhances the efficiency and selectivity of methylation analysis.