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Related Experiment Video

Updated: Jul 10, 2026

Method for Efficient Refolding and Purification of Chemoreceptor Ligand Binding Domain

Method for Efficient Refolding and Purification of Chemoreceptor Ligand Binding Domain

Published on: December 12, 2017

Aptameric enzyme subunit for homogeneous protein sensing.

Wataru Yoshida¹, Koji Sode, Kazunori Ikebukuro

¹Department of Biotechnology, Graduate School of Engineering, Tokyo University of Agriculture & Technology, 2-24-16 Naka-cho, Koganei, Tokyo 184-8588, Japan.

Nucleic Acids Symposium Series (2004)

|November 22, 2007

Summary

A novel aptameric enzyme subunit (AES) enables sensitive detection of immunoglobulin E (IgE). This biosensor utilizes an allosteric mechanism for homogeneous protein sensing with a detection limit of 250 nM.

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Method for Efficient Refolding and Purification of Chemoreceptor Ligand Binding Domain

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Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

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Published on: October 6, 2022

Area of Science:

Biochemistry
Molecular Biology
Analytical Chemistry

Background:

Aptameric enzyme subunits (AES) offer a novel approach for protein sensing by allosterically modulating enzyme activity.
Developing sensitive and specific detection methods for biomarkers like immunoglobulin E (IgE) is crucial for diagnostics.

Purpose of the Study:

To develop and validate an aptameric enzyme subunit (AES) for the detection of immunoglobulin E (IgE).
To investigate the feasibility of using AES in a homogeneous assay format for protein sensing.

Main Methods:

Constructed an AES by integrating an IgE-binding aptamer with a split thrombin-inhibiting aptamer.
Utilized the change in thrombin enzymatic activity, modulated by IgE binding, for detection.
Performed homogeneous assays to quantify IgE levels.

Main Results:

The developed AES demonstrated IgE-dependent inhibition of thrombin enzymatic activity.
The assay achieved a detection limit of 250 nM for IgE.
The AES enabled homogeneous detection of IgE without the need for separation steps.

Conclusions:

The aptameric enzyme subunit (AES) is a viable platform for homogeneous immunoglobulin E (IgE) detection.
This approach offers a sensitive and specific method for protein sensing with potential diagnostic applications.