Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Formation of Complex Ions03:45

Formation of Complex Ions

A type of Lewis acid-base chemistry involves the formation of a complex ion (or a coordination complex) comprising a central atom, typically a transition metal cation, surrounded by ions or molecules called ligands. These ligands can be neutral molecules like H2O or NH3, or ions such as CN− or OH−. Often, the ligands act as Lewis bases, donating a pair of electrons to the central atom. These types of Lewis acid-base reactions are examples of a broad subdiscipline called coordination...
Mismatch Repair01:20

Mismatch Repair

Organisms are capable of detecting and fixing nucleotide mismatches that occur during DNA replication. This sophisticated process requires identifying the new strand and replacing the erroneous bases with correct nucleotides. Mismatch repair is coordinated by many proteins in both prokaryotes and eukaryotes.
The Mutator Protein Family Plays a Key Role in DNA Mismatch Repair
The human genome has more than 3 billion base pairs of DNA per cell. Prior to cell division, that vast amount of genetic...
Mismatch Repair01:36

Mismatch Repair

Overview
Precipitation Titration Curve: Analysis01:21

Precipitation Titration Curve: Analysis

The precipitation titration curve demonstrates the change in concentration of one reactant with the volume of titrant added. During the titration of chloride ions with silver nitrate, the precipitation titration curve is divided into three regions: before, at, and after the equivalence point. Before the equivalence point, low redissolution of the sparingly soluble silver chloride precipitate gives a low silver ion concentration. However, in the second region, representing the equivalence point,...
DNA Base Pairing02:27

DNA Base Pairing

Erwin Chargaff’s rules on DNA equivalence paved the way for the discovery of base pairing in DNA. Chargaff’s rules state that in a double-stranded DNA molecule,
Metal-Ligand Bonds02:51

Metal-Ligand Bonds

The hemoglobin in the blood, the chlorophyll in green plants, vitamin B-12, and the catalyst used in the manufacture of polyethylene all contain coordination compounds. Ions of the metals, especially the transition metals, are likely to form complexes.
In these complexes, transition metals form coordinate covalent bonds, a kind of Lewis acid-base interaction in which both of the electrons in the bond are contributed by a donor (Lewis base) to an electron acceptor (Lewis acid). The Lewis acid in...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Prognostic impact of weight loss during first-line chemotherapy in extensive-disease small cell lung cancer.

Supportive care in cancer : official journal of the Multinational Association of Supportive Care in Cancer·2026
Same author

M-Sec promotes the production of infectious HIV-1 virus through the exocyst complex in macrophages.

PLoS pathogens·2026
Same author

Structural and thermodynamic analyses of a novel β-1,2-glucan binding mode in the ABC transporter solute-binding protein Chy400_4166 from Chloroflexus aurantiacus.

The FEBS journal·2026
Same author

The gp120 Envelope Glycoprotein of HIV-1 Triggers Macropinocytosis in Primary CD4+ T Cells to Promote HIV-1 Infection.

bioRxiv : the preprint server for biology·2026
Same author

Efficacy of Docetaxel Plus Ramucirumab for Malignant Pleural Effusion and Cerebral Edema in Patients With Advanced Non-Small Cell Lung Cancer: A Single-Institution Retrospective Study.

Thoracic cancer·2026
Same author

Weight change and impact on prognosis in patients with advanced non-small cell lung cancer with concomitant diabetes mellitus treated with sglt2 inhibitors.

Supportive care in cancer : official journal of the Multinational Association of Supportive Care in Cancer·2026

Related Experiment Video

Updated: Jul 10, 2026

Ion Mobility-Mass Spectrometry Techniques for Determining the Structure and Mechanisms of Metal Ion Recognition and Redox Activity of Metal Binding Oligopeptides
11:04

Ion Mobility-Mass Spectrometry Techniques for Determining the Structure and Mechanisms of Metal Ion Recognition and Redox Activity of Metal Binding Oligopeptides

Published on: September 7, 2019

The specific interaction between two C:C mismatch base pairs and silver (I) cation.

Hidetaka Torigoe1, Tetsuo Kozasa, Akira Ono

  • 1Department of Applied Chemistry, Faculty of Science, Tokyo University of Science, 1-3 Kagurazaka, Shinjuku-ku, Tokyo 162-8601, Japan. htorigoe@rs.kagu.tus.ac.jp

Nucleic Acids Symposium Series (2004)
|November 22, 2007
PubMed
Summary

Silver (I) cations effectively bind to two C:C mismatch base pairs in DNA heteroduplexes. This binding interaction is consistent regardless of mismatch position, supporting silver cation use for C:C mismatch detection and SNP genotyping.

More Related Videos

Sequence-specific Labeling of Nucleic Acids and Proteins with Methyltransferases and Cofactor Analogues
12:07

Sequence-specific Labeling of Nucleic Acids and Proteins with Methyltransferases and Cofactor Analogues

Published on: November 22, 2014

Quantifying the Binding Interactions Between Cu(II) and Peptide Residues in the Presence and Absence of Chromophores
11:38

Quantifying the Binding Interactions Between Cu(II) and Peptide Residues in the Presence and Absence of Chromophores

Published on: April 5, 2022

Related Experiment Videos

Last Updated: Jul 10, 2026

Ion Mobility-Mass Spectrometry Techniques for Determining the Structure and Mechanisms of Metal Ion Recognition and Redox Activity of Metal Binding Oligopeptides
11:04

Ion Mobility-Mass Spectrometry Techniques for Determining the Structure and Mechanisms of Metal Ion Recognition and Redox Activity of Metal Binding Oligopeptides

Published on: September 7, 2019

Sequence-specific Labeling of Nucleic Acids and Proteins with Methyltransferases and Cofactor Analogues
12:07

Sequence-specific Labeling of Nucleic Acids and Proteins with Methyltransferases and Cofactor Analogues

Published on: November 22, 2014

Quantifying the Binding Interactions Between Cu(II) and Peptide Residues in the Presence and Absence of Chromophores
11:38

Quantifying the Binding Interactions Between Cu(II) and Peptide Residues in the Presence and Absence of Chromophores

Published on: April 5, 2022

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Genetics

Background:

  • Silver (I) cations are known to bind specifically to C:C mismatch base pairs in DNA heteroduplexes.
  • This interaction has been shown to stabilize heteroduplexes, increasing their melting temperatures.

Purpose of the Study:

  • To investigate the thermodynamic properties of silver (I) cation interactions with DNA heteroduplexes containing two C:C mismatch base pairs.
  • To determine the stoichiometry and binding affinity of silver (I) cations to these double mismatches.

Main Methods:

  • Isothermal titration calorimetry (ITC) was employed to analyze the binding of silver (I) cations to synthetic DNA heteroduplexes.
  • Heteroduplexes with varying positions of two C:C mismatch base pairs were synthesized and studied.

Main Results:

  • Two silver (I) cations were found to bind to two C:C mismatch base pairs in DNA heteroduplexes.
  • The binding affinity for the second silver (I) cation was comparable to that of the first, indicating consistent interaction.
  • The thermodynamic parameters and stoichiometry of binding were not significantly affected by the relative positions of the two C:C mismatches.

Conclusions:

  • Silver (I) cation addition is a viable strategy for detecting C:C mismatch base pairs in heteroduplex analysis.
  • These findings may advance the field of single nucleotide polymorphism (SNP) genotyping by improving mismatch detection accuracy.