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Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
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Southern Blot

Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
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In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
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Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes
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Development of high-sensitive DNA probe by using perylene.

Hiromu Kashida1, Tomohiko Takatsu, Hiroyuki Asanuma

  • 1Department of Molecular Design and Engineering, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan.

Nucleic Acids Symposium Series (2004)
|November 22, 2007
PubMed
Summary

Synthesized modified oligodeoxyribonucleotides (ODNs) with perylene moieties enable sensitive detection of one-base deletions. The distinct color change allows for visual identification, simplifying genetic analysis.

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Area of Science:

  • Oligonucleotide Chemistry
  • Molecular Diagnostics
  • Fluorescent Probes

Background:

  • Oligodeoxyribonucleotides (ODNs) are crucial in molecular biology.
  • Accurate detection of genetic variations like single-base deletions is vital for diagnostics.
  • Developing sensitive and accessible detection methods remains a key challenge.

Purpose of the Study:

  • To synthesize novel modified ODNs incorporating perylene moieties.
  • To evaluate the efficacy of these modified ODNs in detecting one-base deletions.
  • To develop a visually detectable assay for genetic variations.

Main Methods:

  • Synthesis of modified oligodeoxyribonucleotides (ODNs) functionalized with two perylene groups.
  • Assay development for detecting one-base deletion mutations using the synthesized ODNs.
  • Spectroscopic analysis to monitor changes in fluorescence emission.

Main Results:

  • Successful synthesis of perylene-modified ODNs.
  • High sensitivity achieved in distinguishing one-base deletion events.
  • Significant and easily observable changes in solution emission color upon successful detection.

Conclusions:

  • Perylene-modified ODNs offer a highly sensitive platform for detecting single-base deletions.
  • The visual color change provides a simple and accessible detection method.
  • This approach has potential applications in genetic analysis and molecular diagnostics.