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Updated: Jul 10, 2026

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

Published on: September 19, 2017

Screen-printed microfluidic device for electrochemical immunoassay.

Hua Dong¹, Chang-Ming Li, Yi-Fan Zhang

¹Center for Advanced Bionanosystems, School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore 637457.

|November 22, 2007

Summary

This summary is machine-generated.

A novel, low-cost microfluidic array device fabricated using screen printing enables simple, mass-producible electrochemical immunoassays. This protein biochip demonstrates high sensitivity and specificity for detecting mouse IgG, promising applications in various fields.

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Area of Science:

Biotechnology
Microfluidics
Biosensors

Background:

Traditional microfabrication is complex and costly.
Need for accessible, mass-producible diagnostic tools.

Purpose of the Study:

To develop a novel microfluidic array device using screen printing technology.
To demonstrate its utility in sandwich-type electrochemical immunoassays.
To showcase its potential for miniaturized, low-cost protein biochips.

Main Methods:

Fabrication of a microfluidic array device using screen printing.
Probe immobilization on electrode surfaces via electropolymerized polypyrrole propylic acid (PPA) film.
Sandwich-type electrochemical immunoassay for mouse IgG detection.

Main Results:

Achieved a detection limit of 10 ng/mL (67 pM) for mouse IgG with a dynamic range of 100 ng/mL-10 μg/mL.
Demonstrated multiplexing capability and high specificity using anti-goat IgG as an alternative probe.
The device enables integrated sample preparation and detection in sealed microchannels.

Conclusions:

The screen-printed microfluidic device offers a simple, inexpensive, and mass-producible platform for electrochemical immunoassays.
The developed system shows great promise for developing miniaturized, low-cost protein biochips for diverse applications.
The device exhibits high sensitivity, specificity, and multiplexing potential.