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09:17
Co-immunoprecipitation Assay Using Endogenous Nuclear Proteins from Cells Cultured Under Hypoxic Conditions
Published on: August 2, 2018
Hypoxia inducible factor (HIF) function in innate immunity and infection.
Annelies S Zinkernagel1, Randall S Johnson, Victor Nizet
1Division of Pediatric Pharmacology & Drug Discovery, University of California, San Diego School of Medicine, Cellular and Molecular Medicine East, Room 1066, 9500 Gilman Drive, MC 0687, La Jolla, CA 92093-0687, USA.
Journal of Molecular Medicine (Berlin, Germany)
|November 22, 2007
Summary
Hypoxia-inducible transcription factor (HIF-1alpha) regulates innate immunity in macrophages and neutrophils. Understanding HIF-1alpha in infections offers new therapeutic strategies for infectious diseases.
Area of Science:
- Immunology
- Cellular Biology
- Molecular Biology
Background:
- Hypoxia-inducible transcription factor (HIF-1alpha) is crucial for cellular adaptation to low oxygen.
- HIF-1alpha regulates energy homeostasis and inflammatory responses.
- Phagocytic cells like macrophages and neutrophils operate in hypoxic environments.
Purpose of the Study:
- To review the role of HIF-1alpha in mammalian innate immunity.
- To highlight insights from conditional gene targeting in myeloid cells.
- To explore HIF-1alpha's impact on host-pathogen dynamics during infection.
Main Methods:
- Literature review of current knowledge on HIF-1alpha.
- Emphasis on studies using conditional gene targeting in myeloid cell lineages.
- Analysis of dynamic HIF-1alpha expression changes during various infections.
Main Results:
- HIF-1alpha acts as a global regulator of macrophage and neutrophil innate immune functions.
- Dynamic changes in HIF-1alpha expression are observed during bacterial, viral, and parasitic infections.
- Conditional gene targeting provides key insights into HIF-1alpha's role in myeloid immunity.
Conclusions:
- HIF-1alpha plays a significant role in the innate immune response to infections.
- Understanding HIF-1alpha function can inform the development of novel therapies for infectious diseases.
- Targeting HIF-1alpha may offer rational approaches to enhance innate immune function.
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