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Related Experiment Video

Updated: Jul 10, 2026

Localization of SUMO-modified Proteins Using Fluorescent Sumo-trapping Proteins

Localization of SUMO-modified Proteins Using Fluorescent Sumo-trapping Proteins

Published on: April 27, 2019

SUMO: getting it on.

J Anckar¹, L Sistonen

¹Turku Centre for Biotechnology, University of Turku and Abo Akademi University, PO Box 123, FI-20521 Turku, Finland.

Biochemical Society Transactions

|November 23, 2007

Summary

This summary is machine-generated.

Small ubiquitin-related modifier (SUMO) conjugation is a key protein regulation. Extended motifs, beyond consensus sites, provide crucial specificity for SUMOylation, influencing biological processes.

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SUMO-Binding Entities (SUBEs) as Tools for the Enrichment, Isolation, Identification, and Characterization of the SUMO Proteome in Liver Cancer

SUMO-Binding Entities (SUBEs) as Tools for the Enrichment, Isolation, Identification, and Characterization of the SUMO Proteome in Liver Cancer

Published on: November 1, 2019

Area of Science:

Molecular Biology
Biochemistry
Cell Biology

Background:

SUMOylation is a critical post-translational modification regulating diverse cellular processes.
SUMO conjugation exhibits high specificity for target lysine residues, yet selection mechanisms remain incompletely understood.
While Ubc9 (ubiquitin-conjugating enzyme 9) catalyzes SUMOylation, not all consensus sites are modified, indicating additional regulatory factors.

Purpose of the Study:

To explore the role of extended motifs in regulating SUMO conjugation.
To investigate specificity determinants beyond the canonical PsiKXE SUMO consensus motif.
To understand how other post-translational modifications influence SUMOylation site selection.

Main Methods:

Review of existing literature on SUMOylation and protein modification.
Analysis of known SUMOylation consensus motifs (PsiKXE) and their limitations.
Examination of the interplay between SUMOylation, phosphorylation, and acetylation.

Main Results:

SUMOylation specificity is not solely determined by the Ubc9-binding PsiKXE motif.
Extended sequence contexts, including negatively charged residues and phosphorylation, dictate SUMOylation site selection.
Regulation of SUMOylation is intricately linked with other post-translational modifications.

Conclusions:

Extended motifs play a significant role in conferring specificity to SUMOylation.
Understanding these extended motifs is crucial for a comprehensive view of SUMOylation regulation.
This knowledge advances our understanding of how protein function is modulated through SUMOylation.