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Updated: Jul 10, 2026

A Simple Fluorescence-based Reporter Assay to Identify Cellular Components Required for Ricin Toxin A Chain (RTA) Trafficking in Yeast
Published on: December 15, 2017
Zymocin, a composite chitinase and tRNase killer toxin from yeast
1Biologicum, Institut für Biologie, Bereich Genetik, Martin-Luther-Universität Halle-Wittenberg, Weinbergweg 10, D-06120 Halle/Saale, Germany.
Abstract:
Growth inhibition of Saccharomyces cerevisiae by the plasmid-encoded trimeric (alphabetagamma) zymocin toxin from dairy yeast, Kluyveromyces lactis, depends on a multistep response pathway in budding yeast. Following early processes that mediate cell-surface contact by the chitinase alpha-subunit of zymocin, later steps enable import of the gamma-toxin tRNase subunit and cleavage of target tRNAs that carry modified U34 (wobble uridine) bases. With the emergence of zymocin-like toxins, continued zymocin research is expected to yield new insights into the evolution of yeast pathosystems and their lethal modes of action.
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