Related Experiment Video
Updated: Jul 9, 2026

A Rapid, Scalable Method for the Isolation, Functional Study, and Analysis of Cell-derived Extracellular Matrix
Published on: January 4, 2017
The role of GlcNAc in formation and function of extracellular matrices
1Max-Planck-Institut für Entwicklungsbiologie, Spemannstr. 35, 72076 Tübingen, Germany. bernard.moussian@tuebingen.mpg.de
Abstract:
N-acetylglucosamine (GlcNAc) is an abundant hexose that as a monomer or as part of macromolecules plays multiple roles in eukaryotic cells. Especially as a residue of oligo- and polysaccharides and conjugated with lipids and proteins, GlcNAc contributes to the function and architecture of extracellular matrices. Several human disorders caused by mutations in genes coding for enzymes dealing with GlcNAc have been identified. However, molecular aspects of their clinical picture are laborious to investigate and are mostly addressed in yeast and vertebrate cultured cells that as unicellular or artificial systems ultimately do not allow conclusive deductions for complex organisms. An excellent model system to study the biology of GlcNAc in a multi-cellular organism is the genetically, biochemically and physiologically manipulable fruitfly Drosophila melanogaster that despite the evolutionary distance shares basic features of GlcNAc biology with humans. The aim of this review is to summarise the use of GlcNAc both in mammals and in Drosophila by highlighting the molecular consequences of perturbing GlcNAc function.
Related Concept Videos
The Extracellular Matrix
In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.
Composition of the Extracellular Matrix
The extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse...
The Extracellular Matrix
Overview of Cell-Matrix Interactions
Glycosaminoglycans
GAGS are found in the extracellular matrix of vertebrates, invertebrates, and bacteria. Due to their polar nature they attract water, and serve as excellent lubricants or shock absorbers in an animal body.
Hyaluronic...
Extracellular Matrix
Matrix Proteoglycans and Glycoproteins
