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High-throughput Detection Method for Influenza Virus
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Rapid and highly sensitive coxsackievirus a indirect immunofluorescence assay typing kit for enterovirus serotyping

Tsuey-Li Lin1, Yi-Syue Li, Chiao-Wei Huang

  • 1Center for Research and Diagnostic, Centers for Disease Control, Department of Health, Taiwan, 161 Kunyang Street, Nan-Kang, Taipei, Taiwan.

Journal of Clinical Microbiology
|November 23, 2007
PubMed

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Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.