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Restriction map of the Casphalia extranea densovirus genome
G Fédière1, S Herder, K N Kouassi
1Laboratoire d'Entomovirologie ORSTOM, BPV 51 Abidjan Côte-d'Ivoire.
Abstract:
A physical map of the Casphalia extranea densovirus genome (CeDNV) was constructed. The size of the intact viral genome was estimated to be 4.9 kilobases or 1.6 MDa (single strand). The double-stranded CeDNV genomic DNA was cleaved with 26 restriction endonucleases and 20 restriction sites were mapped on the genome. The CeDNV DNA restriction map was compared to those of other densoviruses. Southern blotting hybridization experiments failed to reveal any homology between the genomes of CeDNV and Junoniacoenia densovirus (JcDNV).
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