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Chilling induces a decrease in pyrophosphate-dependent H+-accumulation associated with a DeltapH(vac)-stat in mung
1Department of Cell Biology and Molecular Genetics, University of Maryland, HJ Patterson Hall, College Park, MD, USA. ykawa@iwate-u.ac.jp
Plant, Cell & Environment
|November 24, 2007
Summary
Chilling causes cytoplasmic acidification in sensitive plants by disrupting the vacuolar pH stat. Pyrophosphate-dependent proton accumulation, crucial for maintaining this pH balance, is impaired by cold stress.
Area of Science:
- Plant Physiology
- Cell Biology
- Biochemistry
Background:
- Chilling stress in plants can lead to cytoplasmic acidification.
- A potential mechanism involves the disruption of the vacuolar pH-stat (DeltapH(vac)-stat).
Purpose of the Study:
- To investigate the role of temperature on vacuolar acidification mechanisms.
- To understand how chilling affects pyrophosphate (PPi)- and ATP-dependent proton (H+) transport in mung bean vacuolar vesicles.
Main Methods:
- Isolated vacuolar vesicles from mung bean hypocotyls.
- Measured H+ influx and efflux mediated by pyrophosphatase (PPase) and PPi-dependent suppression.
- Assessed the effect of temperature (20°C to 0°C) on vesicle acidification.
- Compared vesicles from control and cold-stressed (0°C for 1 day) seedlings.
Main Results:
- PPi-dependent H+ accumulation maintained a constant internal vesicle pH (pH(in) ≈ 5) against temperature changes.
- ATP-dependent DeltapH(in) decreased with dropping temperatures.
- Chilling reduced PPi-dependent H+ accumulation by increasing H+ efflux and decreasing H+ influx.
- Chilling led to a lower steady-state DeltapH(in) mediated by PPi.
Conclusions:
- PPi-dependent H+ accumulation is vital for maintaining the vacuolar pH-stat against temperature fluctuations.
- Impaired PPi-dependent H+ accumulation due to chilling contributes to cytoplasmic acidification in sensitive plants.
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