Related Experiment Video
Updated: Jul 9, 2026

Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification
Published on: September 21, 2011
Characterization and applications of reversed-phase column selectivity based on the hydrophobic-subtraction model
D H Marchand1, L R Snyder, J W Dolan
1University of Wisconsin-River Falls, River Falls, WI 54022-5001, USA. daniel.marchand@uwrf.edu
Abstract:
A total of 371 reversed-phase columns have now been characterized in terms of selectivity, based on five solute-column interactions (the hydrophobic-subtraction model). The present study illustrates the use of these data for interpreting peak-tailing and column stability. New insights are also provided concerning column selectivity as a function of ligand and silica type, and the selection of columns for orthogonal separations is re-examined. Some suggestions for the quality control of reversed-phase columns during manufacture are offered.
Related Concept Videos
Ion-Exchange Chromatography
High-Performance Liquid Chromatography: Introduction
In HPLC, two phases play a critical role in the separation process:
Size-Exclusion Chromatography
Silica particles offer advantages such as rigidity,...
Affinity Chromatography
Chromatography: Introduction
The phase in which the compounds linger or on which the compounds adsorb is called the stationary phase, whereas the mobile phase is the solvent that carries the solutes to be analyzed. In traditional column chromatography, the mixture flows through the stationary phase, and the compounds partition between the stationary and mobile phases...
Ion Exchange

