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Related Experiment Videos

Determining blood cell size using microfluidic hydrodynamics.

David W Inglis1, John A Davis, Thomas J Zieziulewicz

  • 1Princeton Institute for the Science and Technology of Materials (PRISM), Department of Electrical Engineering, Princeton University, Princeton, NJ 08544, USA. dinglis@ics.mq.edu.au

Journal of Immunological Methods
|November 27, 2007
PubMed
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A novel microfluidic device measures cell size using hydrodynamic displacement, offering comparable resolution to conventional flow cytometry. This technology enables cell differentiation and activation detection with reduced cost and complexity.

Area of Science:

  • Biotechnology
  • Biomedical Engineering
  • Cell Biology

Background:

  • Conventional flow cytometry and FACS analyze limited parameters in microfluidic devices due to cost and complexity.
  • There is a need for microfluidic devices capable of analyzing more cell parameters with minimal resources.

Purpose of the Study:

  • To introduce a novel microfluidic parameter, "hydrodynamic" cell size.
  • To compare the performance of microfluidic hydrodynamic size measurement with conventional forward scatter.
  • To demonstrate the utility of this microfluidic device for cell differentiation and activation detection.

Main Methods:

  • Development of a microfluidic device with an array of micro-fabricated obstacles (1.2 mm wide).
  • Measurement of "hydrodynamic" cell size based on lateral displacement through the obstacle array.

Related Experiment Videos

  • Comparison of microfluidic hydrodynamic size with forward scatter from conventional flow cytometry.

    • Main Results:

    • Comparable size resolution was achieved between the microfluidic device and conventional forward scatter.
    • The microfluidic device successfully differentiated healthy and malignant lymphocytes by size alone.
    • Increased numbers of activated lymphocytes post-exposure to staphylococcal enterotoxin B (SEB) were detected.

    Conclusions:

    • The microfluidic device provides a new parameter, "hydrodynamic" cell size, for cell analysis.
    • This technology offers comparable performance to conventional flow cytometry for certain applications.
    • The device presents a potentially lower-cost and less complex alternative for flow cytometry tasks.