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Related Experiment Videos

Label-free quantitative DNA detection using the liquid core optical ring resonator.

Jonathan D Suter1, Ian M White, Hongying Zhu

  • 1Department of Biological Engineering, 240D Bond Life Sciences Center, University of Missouri-Columbia, Columbia, MO 65211, USA.

Biosensors & Bioelectronics
|November 27, 2007
PubMed
Summary
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This study showcases the liquid core optical ring resonator (LCORR) sensor for quantitative, real-time, label-free DNA sequence detection. The LCORR sensor accurately differentiates DNA sequences, even with mismatches, and achieves low detection limits for DNA analysis.

Area of Science:

  • Biophotonics
  • Nanotechnology
  • Molecular Diagnostics

Background:

  • Label-free biosensing is crucial for rapid molecular diagnostics.
  • Optical ring resonators offer high sensitivity for detecting biomolecular interactions.
  • Microfluidics integration enhances sample handling and detection volume efficiency.

Purpose of the Study:

  • To demonstrate quantitative, real-time, label-free DNA sequence detection using a liquid core optical ring resonator (LCORR) sensor.
  • To evaluate the LCORR sensor's capability in detecting DNA sequences with varying lengths, mismatches, and concentrations.
  • To establish a foundation for developing LCORR-based DNA label-free microarrays.

Main Methods:

  • Utilized a liquid core optical ring resonator (LCORR) sensor integrated with microfluidics.

Related Experiment Videos

  • Experimentally and theoretically analyzed LCORR response to diverse DNA samples (25-100 bases, 1-5 mismatches, 10 pM to 10 microM).
  • Established linear correlation between LCORR signal and molecule density for accurate quantification.

    • Main Results:

    • Achieved quantitative real-time label-free DNA detection with a low detection limit of 10 pM.
    • Demonstrated accurate differentiation of DNA sequences with 1-5 base mismatches.
    • Determined probe surface coverage (26-51%), hybridization extent (40-50%), and a dissociation constant of 2.9 nM for a 25-base DNA pair.
    • Estimated mass detection limit of 4 pg/mm², equivalent to 10^10 molecules/cm².

    Conclusions:

    • The LCORR sensor provides a sensitive and accurate platform for label-free DNA sequence detection and mismatch differentiation.
    • Established a quantitative relationship between LCORR signal and DNA molecule density.
    • The findings support the potential of LCORR technology for developing advanced DNA microarrays and diagnostic tools.