Related Experiment Video
Updated: Jul 9, 2026

Gene Transfection toward Spheroid Cells on Micropatterned Culture Plates for Genetically-modified Cell Transplantation
Published on: July 31, 2015
Genetic modification of cells for transplantation
Yi Lai1, Irina Drobinskaya, Eugen Kolossov
1Department of Molecular Microbiology & Immunology, University of Missouri-Columbia, USA.
Abstract:
Progress in gene therapy has produced promising results that translate experimental research into clinical treatment. Gene modification has been extensively employed in cell transplantation. The main barrier is an effective gene delivery system. Several viral vectors were utilized in end-stage differentiated cells. Recently, successful applications were described with adenovirus-associated vectors. As an alternative, embryonic stem cell- and stem cell-like systems were established for generation of tissue-specified gene-modified cells. Owing to the feasibility for genetic manipulations and the self-renewing potency of these cells they can be used in a way enabling large-scale in vitro production. This approach offers the establishment of in vitro cell culture systems that will deliver sufficient amounts of highly purified, immunoautologous cells suitable for application in regenerative medicine. In this review, the current technology of gene delivery systems to cells is recapitulated and the latest developments for cell transplantation are discussed.
Related Concept Videos
Methods of Nuclear Reprogramming
Tissue Transplantation
The Biology of Tissue Transplantation
The biology of tissue transplantation hinges on the Major Histocompatibility Complex (MHC) molecules. These molecules...
iPS Cell Differentiation
Induced Pluripotent Stem Cells
Somatic cells are...
Introduction to Nuclear Reprogramming
Somatic to iPS Cell Reprogramming

