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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
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Rescue of Recombinant Newcastle Disease Virus from cDNA
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Published on: October 11, 2013

[Important points in virus research using recombinant DNA technology].

Takahiko Nikaido1, Kaoru Takeuchi

  • 1Life Science Division, Research Promotion Bureau, Ministry of Education, Culture, Sports, Science and Technology of Japan, 2-5-1 Marunouchi, Chiyoda-ku, Tokyo 100-8959, Japan. tnikaido@mext.go.jp

Uirusu
|November 28, 2007
PubMed
Summary
This summary is machine-generated.

The Cartagena Protocol protects biodiversity from genetically modified organisms. Japan

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Area of Science:

  • Biosafety
  • Biotechnology
  • Environmental Law

Context:

  • The Cartagena Protocol on Biosafety aims to safeguard biological diversity from risks associated with living modified organisms (LMOs) developed through modern biotechnology.
  • Japan ratified the protocol, implementing domestic legislation and regulations to align with its objectives.

Purpose:

  • To explain the Cartagena Protocol on Biosafety and Japan's domestic regulations concerning LMOs.
  • To clarify the classification of LMO use in Japan (Type 1 and Type 2) and the approval process for experiments involving recombinant viruses.

Summary:

  • Japan classifies LMO use into Type 1 (no containment) and Type 2 (containment measures).
  • Most experiments using recombinant viruses require ministerial approval under Japanese domestic law.
  • The article provides an overview of the protocol, Japanese law, and an example application form.

Impact:

  • Ensures compliance with international biosafety standards in Japan.
  • Facilitates understanding of regulatory requirements for researchers working with LMOs in Japan.
  • Promotes safe application of modern biotechnology while protecting biodiversity.