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Related Experiment Video

Updated: Jul 9, 2026

Characterization of Human Monocyte-derived Dendritic Cells by Imaging Flow Cytometry: A Comparison between Two Monocyte Isolation Protocols
08:31

Characterization of Human Monocyte-derived Dendritic Cells by Imaging Flow Cytometry: A Comparison between Two Monocyte Isolation Protocols

Published on: October 18, 2016

Methamphetamine modulates DC-SIGN expression by mature dendritic cells.

Madhavan P N Nair1, Supriya Mahajan, Don Sykes

  • 1Department of Medicine, Division of Allergy, Immunology, and Rheumatology, State University of New York and Buffalo General Hospital, Kaleida Health System, 100 High Street, Buffalo, NY 14203, USA. mnair@acsu.buffalo.edu

Journal of Neuroimmune Pharmacology : the Official Journal of the Society on Neuroimmune Pharmacology
|November 28, 2007
PubMed
Summary

Methamphetamine may worsen HIV-1 by increasing dendritic cell (DC)-specific intercellular adhesion molecule-3 grabbing non-integrin (DC-SIGN) on DCs. This effect is mediated by dopamine D1 receptors and reversed by antagonists.

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Area of Science:

  • Immunology
  • Virology
  • Neuropharmacology

Background:

  • Methamphetamine (meth) use is associated with increased HIV-1 progression.
  • Dendritic cells (DCs) play a crucial role in HIV-1 pathogenesis.
  • DC-SIGN on DCs is implicated in HIV-1 binding and transmission.

Purpose of the Study:

  • To investigate the effect of methamphetamine on DC-SIGN expression in mature dendritic cells (mDCs).
  • To elucidate the role of dopamine D1 receptors in mediating meth-induced changes in DC-SIGN expression.
  • To explore the synergistic effect of meth and HIV-1 gp120 on DC-SIGN expression.

Main Methods:

  • Culture of mature dendritic cells (mDCs) from normal subjects.
  • Treatment of mDCs with methamphetamine (meth).
  • Analysis of DC-SIGN gene and protein expression using real-time quantitative PCR and fluorescence-activated cell sorting (FACS).
  • Assessment of meth-induced effects using a dopamine D1 receptor antagonist (SCH 23390) and D1 receptor-specific small interfering RNA (siRNA).
  • Evaluation of the combined effect of meth and HIV-1 peptide gp120.

Main Results:

  • Methamphetamine significantly up-regulated both DC-SIGN gene and protein expression in mDCs.
  • The meth-induced up-regulation of DC-SIGN was reversed by the dopamine D1 receptor antagonist SCH 23390 and D1R-specific siRNA, indicating mediation via D1 receptors.
  • Methamphetamine, in synergy with HIV-1 peptide gp120, further enhanced DC-SIGN gene expression in mDCs.

Conclusions:

  • Methamphetamine up-regulates DC-SIGN expression on mature dendritic cells.
  • Dopamine D1 receptors mediate the effects of methamphetamine on DC-SIGN expression.
  • These findings suggest a potential mechanism by which methamphetamine exacerbates HIV-1 pathogenesis in drug users.
  • Understanding DC-SIGN's role could lead to novel therapeutic strategies for HIV-1 in this population.