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Cytoplasmic proteome reference map for a glutamic acid-producing Corynebacterium glutamicum ATCC 14067
Liyuan Li1, Masaru Wada, Atsushi Yokota
1Laboratory of Microbial Physiology, Division of Applied Bioscience, Research Faculty of Agriculture, Hokkaido University, Hokkaido, Japan.
Proteomics
|November 28, 2007
Summary
We created a protein map for glutamic acid-producing Corynebacterium glutamicum. Strain-specific maps are crucial for accurate proteomic analysis, as significant differences were observed between strains.
Area of Science:
- Microbiology
- Proteomics
- Biotechnology
Background:
- Corynebacterium glutamicum is a key industrial microorganism for amino acid production.
- Proteomic analysis is essential for understanding cellular functions and optimizing production strains.
- A comprehensive cytoplasmic proteome reference map is needed for strain ATCC 14067.
Purpose of the Study:
- To construct a cytoplasmic proteome reference map for Corynebacterium glutamicum ATCC 14067.
- To identify proteins in the cytoplasmic proteome of this strain.
- To compare proteomic profiles with a related strain.
Main Methods:
- Two-dimensional gel electrophoresis (2-DE) was employed to separate cytoplasmic proteins.
- Protein spots were identified using matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS).
- Peptide mass fingerprinting (PMF) and the genome database of strain ATCC 13032 were used for protein identification.
Main Results:
- A reference map identified 166 protein spots, representing 139 distinct proteins.
- Significant differences in proteomic images were observed between C. glutamicum ATCC 14067 and ATCC 13032.
- These differences were noted under glutamic acid production conditions.
Conclusions:
- The study successfully generated a cytoplasmic proteome reference map for C. glutamicum ATCC 14067.
- Proteomic analysis revealed considerable strain-specific variations.
- A strain-specific reference map is vital for accurate proteomic investigations in C. glutamicum.
