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Updated: Jul 9, 2026
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Array Comparative Genomic Hybridization (Array CGH) for Detection of Genomic Copy Number Variants
Published on: February 21, 2015
Evaluation of whole genome amplification protocols for array and oligonucleotide CGH
Adam Hittelman1, Shivaranjani Sridharan, Ritu Roy
1Department of Urology University of California at San Francisco Comprehensive Cancer Center, San Francisco, CA, USA.
Summary
Whole genome amplification is crucial for clinical oncology. Sigma
Area of Science:
- Genomic analysis in clinical oncology
- Molecular diagnostics and personalized medicine
Background:
- Genome-based technologies like genomic arrays and next-generation sequencing are vital for clinical oncology.
- Translating these technologies requires high-quality, reproducible data from small, archived tumor specimens and biopsies.
Purpose of the Study:
- To systematically compare multiple whole genome amplification (WGA) methods.
- To evaluate WGA performance on various challenging clinical specimens, including formalin-fixed, paraffin-embedded (FFPE) tissues.
Main Methods:
- Comprehensive microarray analysis.
- Quantitative analysis and clustering of WGA results.
- Testing on diverse clinical specimens, including FFPE biopsies.
Main Results:
- Sigma's whole genome amplification protocol demonstrated superior performance across all tested specimens.
- The Sigma protocol was effective even with difficult formalin-fixed, paraffin-embedded biopsy samples.
Conclusions:
- Sigma's WGA method is highly suitable for clinical oncology applications.
- This protocol enables reliable genomic analysis from challenging clinical samples, facilitating translation to patient care.
