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252Cf-plasma desorption and cesium-ion liquid secondary-ion mass spectrometric analysis of recombinant proteins
A Tsarbopoulos1, B N Pramanik, P Reichert
1American Cyanamid Company, Lederle Laboratories, Pearl River, NY 10965.
Rapid Communications in Mass Spectrometry : RCM
|February 1, 1991
Abstract:
The 35 keV Cs+ liquid secondary-ion mass spectrometry (LSIMS) and 252Cf-plasma desorption (PD) mass spectra of recombinant proteins in the 10-25 kDa mass range are compared. Both techniques showed comparable mass accuracy and sensitivity, and in the case of LSIMS, remarkably short analysis time. Analysis by the PD/nitrocellulose method demonstrated slightly higher sensitivity and relatively lower dependence on the salt and buffer content of the protein sample.