Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Imaging Biological Samples with Optical Microscopy

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...

Electron Microscope Tomography and Single-particle Reconstruction

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...

Two-Dimensional Microscopy in Microbiology

Two-Dimensional Microscopy in Microbiology

Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...

Three-Dimensional Microscopy in Microbiology

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

Phase Contrast and Differential Interference Contrast Microscopy

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Widespread use of invalid statistical tests in biomedical machine learning.

bioRxiv : the preprint server for biology·2026

Same author

Don't predict if you cannot interpret: investigating the clinical viability of facial movements for machine-learning assisted diagnostics of bipolar disorder.

Nordic journal of psychiatry·2026

Same author

[Transitioning to digital pathology: Feedback from the eNovA-Path project of the 3 university hospitals of Nouvelle Aquitaine (Poitiers, Limoges and Bordeaux)].

Annales de pathologie·2025

Same author

Do you look longer at attractive faces? It depends on what you are looking for.

i-Perception·2024

Same author

Acceleration of cutaneous T-cell lymphoma mistaken for atopic dermatitis following JAK inhibitor use.

European journal of dermatology : EJD·2024

Same author

MuscleJ2: a rebuilding of MuscleJ with new features for high-content analysis of skeletal muscle immunofluorescence slides.

Skeletal muscle·2023

Search research articles

Related Experiment Video

Updated: Jul 9, 2026

Imaging Intermediate Filaments and Microtubules with 2-dimensional Direct Stochastic Optical Reconstruction Microscopy

Imaging Intermediate Filaments and Microtubules with 2-dimensional Direct Stochastic Optical Reconstruction Microscopy

Published on: March 6, 2018

A Bayesian reconstruction method for micro-rotation imaging in light microscopy.

Danai Laksameethanasan¹, Sami S Brandt, Peter Engelhardt

¹Laboratory of Computational Engineering, Helsinki University of Technology, FI-02015 TKK, Finland. danai.laksameethanasan@tkk.fi

Microscopy Research and Technique

|November 30, 2007

Summary

This study introduces a novel 3D reconstruction algorithm and deblurring method for light microscopy utilizing micro-rotation imaging. The developed technique offers a promising approach for enhanced 3D imaging and deblurring in microscopy applications.

More Related Videos

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

Published on: November 16, 2019

Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins

Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins

Published on: May 3, 2022

Related Experiment Videos

Last Updated: Jul 9, 2026

Imaging Intermediate Filaments and Microtubules with 2-dimensional Direct Stochastic Optical Reconstruction Microscopy

Imaging Intermediate Filaments and Microtubules with 2-dimensional Direct Stochastic Optical Reconstruction Microscopy

Published on: March 6, 2018

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

Published on: November 16, 2019

Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins

Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins

Published on: May 3, 2022

Area of Science:

Microscopy and Imaging Technologies
Computational Imaging
Optical Physics

Background:

Conventional 3D optical imaging relies on shifting the focal plane along the optical axis.
Micro-rotation imaging offers an alternative 3D imaging modality by rotating the object within a fixed optical setup using dielectric fields.

Purpose of the Study:

To develop a 3D reconstruction algorithm for the novel micro-rotation imaging modality.
To create a reconstruction-based deblurring method to remove out-of-focus light from micro-rotation image series.
To analyze the strengths and weaknesses of micro-rotation imaging compared to conventional methods.

Main Methods:

A reconstruction algorithm based on Bayesian inversion theory was developed.
The total variation function was employed as a structure prior in the reconstruction algorithm.
Simulations were used to study the spectral properties and performance of the micro-rotation approach.
The method was validated using real data sets.

Main Results:

The developed algorithm successfully performed 3D reconstruction from micro-rotation image series.
The method demonstrated effectiveness as a deblurring technique for removing out-of-focus light.
Simulations provided insights into the comparative performance of micro-rotation versus conventional 3D imaging.

Conclusions:

The presented micro-rotation imaging approach is a promising technique for 3D reconstruction in light microscopy.
The reconstruction-based deblurring method effectively removes out-of-focus light, enhancing image quality.
This work lays the foundation for further development and application of micro-rotation imaging in scientific research.