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Updated: Jul 9, 2026

Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions
Published on: August 3, 2021
Absence of ethanol-induced interdigitation in supported phospholipid bilayers on silica surfaces
Adam Miszta1, Bas van Deursen, Roy Schoufs
1J. Heyrovsky Institute of Physical Chemistry v.v.i., Academy of Sciences of the Czech Republic, Dolejskova 3, Prague, Czech Republic.
Abstract:
Membranes prepared by the adsorption of phospholipid vesicles on solid supports are much-used model systems in biomedical research. However, there is accumulating evidence that such membranes may not always be equivalent to the free-standing cellular membranes that they are modeling. In the present study, sonicated DOPC/DOPS (80/20 mol %) vesicles were adsorbed on hydrophilic silica surfaces, a system that has been demonstrated to produce confluent bilayers. In addition, pure DOPC and DLPC membranes were studied. It is demonstrated that ethanol-induced membrane interdigitation, as demonstrated for free-standing bilayers, does not occur in these supported membranes.
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