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Characterization of dehydromonacolin-MV2 from Monascus purpureus mutant
M A Dhale1, S Divakar, S Umesh-Kumar
1Department of Food Microbiology, Central Food Technological Research Institute, Mysore-570 020, India.
Aim:
Characterization of dehydromonacolin-MV2, a bioactive metabolite isolated from Monascus purpureus mutant (CFR 410-11).
Methods And Results:
Chloroform extract of rice, fermented with a hyperpigment-producing mutant of M. purpureus (CFR 410-11) was found to contain metabolites that inhibited the growth of Bacillus, Pseudomonas and Streptococcus in agar gel diffusion assays. The extract inhibited lipid peroxidation and scavenged 2,2-diphenyl-1-pycrylhydrazyl and hydroxyl radicals. The active compound purified by silica gel column chromatography was characterized by NMR. The carbon, proton and 2D HSQCT assignments identified dehydromonacolin-MV2 as the bioactive metabolite.
Conclusions:
Dehydromonacolin-MV2 apparently originated in the mutant by hydroxylation and oxidation of monacolin-J, an intermediate of monacolin biosynthetic pathway.
Significance And Impact Of The Study:
Identification of the production of dehydromonacolin-MV2 by M. purpureus mutant (CFR 410-11) is new to literature. Bioactive properties of the compound suggested its pharmaceutical applications.
Insights
A novel bioactive metabolite, dehydromonacolin-MV2, was identified from a Monascus purpureus mutant. This compound exhibits antimicrobial and antioxidant properties, suggesting potential pharmaceutical applications.
Area of Science:
- Microbiology
- Natural Product Chemistry
- Biochemistry
Background:
- Monascus purpureus is known for producing bioactive metabolites.
- Mutant strains can yield novel compounds with enhanced properties.
- Characterizing new metabolites is crucial for understanding their biological roles.
Purpose of the Study:
- To isolate and characterize a novel bioactive metabolite from a Monascus purpureus mutant (CFR 410-11).
- To investigate the antimicrobial and antioxidant activities of the isolated compound.
- To elucidate the biosynthetic origin of the metabolite.
Main Methods:
- Fermentation of M. purpureus mutant (CFR 410-11) on rice.
- Extraction and purification using silica gel column chromatography.
- Structural elucidation using Nuclear Magnetic Resonance (NMR) spectroscopy (1D and 2D).
- Antimicrobial activity assessed by agar gel diffusion assays.
- Antioxidant activity evaluated through lipid peroxidation inhibition and radical scavenging assays.
Main Results:
- A chloroform extract demonstrated significant inhibition against Bacillus, Pseudomonas, and Streptococcus species.
- The extract exhibited potent antioxidant activity, including scavenging of DPPH and hydroxyl radicals.
- NMR analysis identified the active compound as dehydromonacolin-MV2.
- Dehydromonacolin-MV2 was found to inhibit lipid peroxidation.
Conclusions:
- Dehydromonacolin-MV2 is a newly identified bioactive metabolite produced by M. purpureus mutant CFR 410-11.
- The compound likely originates from the hydroxylation and oxidation of monacolin-J.
- The observed antimicrobial and antioxidant properties suggest potential pharmaceutical applications for dehydromonacolin-MV2.
