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Lipopolysaccharide induced expression of pentraxin 3 in human neutrophils and monocyte-derived macrophages

Masaru Imamura1, Takashi Kawasaki, Alexander S Savchenko

  • 1Division of Cellular and Molecular Pathology, Department of Cellular Function, Niigata University Graduate School of Medical and Dental Sciences, Asahimachi 1-757, Chuou-ku, Niigata 951-8510, Japan.

Cellular Immunology
|November 30, 2007
PubMed

Insights

Pentraxin 3 (PTX3) is expressed in monocytes/macrophages and neutrophils during inflammation. PTX3 levels increase after lipopolysaccharide (LPS) stimulation, suggesting its potential as a diagnostic tool for peripheral inflammation.

Area of Science:

  • Immunology
  • Biochemistry
  • Cell Biology

Background:

  • Pentraxin 3 (PTX3) is a long pentraxin protein.
  • PTX3 is produced by monocytes/macrophages (Mphis) and other cells in response to inflammatory stimuli like lipopolysaccharide (LPS).

Purpose of the Study:

  • To investigate PTX3 expression in human monocyte-derived Mphis and neutrophils.
  • To determine the role of PTX3 in inflammatory responses.

Main Methods:

  • Immunoblotting
  • Immunohistochemical staining
  • Reverse transcriptase-polymerase chain reaction (RT-PCR)
  • Quantitative real-time PCR
  • Enzyme-linked immunosorbent assay (ELISA)

Main Results:

  • PTX3 expression was detected in the cytoplasm of both GM-CSF and M-CSF induced Mphis.
  • PTX3 levels were upregulated in Mphis 24 hours after LPS stimulation.
  • PTX3 was expressed in freshly isolated neutrophils and distinctly upregulated at 6 and 24 hours post-LPS stimulation.

Conclusions:

  • PTX3 expression in both Mphis and neutrophils indicates its involvement in inflammation.
  • PTX3 may serve as a valuable diagnostic marker for assessing inflammation at peripheral sites.

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