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Updated: Jul 9, 2026

Isolation and Differentiation of Primary White and Brown Preadipocytes from Newborn Mice
09:00

Isolation and Differentiation of Primary White and Brown Preadipocytes from Newborn Mice

Published on: January 25, 2021

Conditionally immortalized white preadipocytes: a novel adipocyte model.

D L Morganstein1, M Christian, J J O Turner

  • 1Institute of Reproductive and Developmental Biology, Imperial College London, London W12 0NN, United Kingdom.

Journal of Lipid Research
|November 30, 2007
PubMed
Summary

Researchers developed a new method to create conditionally immortalized white adipocyte cell lines (IMWAT). These cells mimic white fat cells and are useful for studying adipocyte function and gene regulation.

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Area of Science:

  • Biochemistry
  • Cell Biology
  • Metabolic Research

Background:

  • Adipocyte research is crucial for understanding metabolic diseases.
  • Primary adipocytes have limitations for extensive study.
  • Developing robust cell models is essential for adipocyte research.

Purpose of the Study:

  • To establish a novel method for generating conditionally immortalized preadipocyte cell lines from white adipose tissue (IMWAT).
  • To create a versatile cell model for investigating adipocyte function and gene regulation.

Main Methods:

  • Generation of conditionally immortalized preadipocyte cell lines from white adipose tissue.
  • Induction of differentiation into white adipocytes.
  • Characterization of adipocyte markers, insulin signaling, and cytokine expression.

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Isolation and Differentiation of Stromal Vascular Cells to Beige/Brite Cells
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Last Updated: Jul 9, 2026

Isolation and Differentiation of Primary White and Brown Preadipocytes from Newborn Mice
09:00

Isolation and Differentiation of Primary White and Brown Preadipocytes from Newborn Mice

Published on: January 25, 2021

Isolation and Differentiation of Stromal Vascular Cells to Beige/Brite Cells
07:22

Isolation and Differentiation of Stromal Vascular Cells to Beige/Brite Cells

Published on: March 28, 2013

  • Utilizing adenoviral vectors and short hairpin RNA for gene manipulation.

    • Main Results:

    • IMWAT cells successfully differentiate into white adipocytes expressing key markers (PPARγ, aP2).
    • These adipocytes maintain an intact insulin signaling pathway and express proinflammatory cytokines.
    • IMWAT cells are amenable to genetic manipulation, enabling functional studies.
    • Depletion of RIP140 in IMWAT cells mimics metabolic gene expression changes seen in RIP140 null mice.

    Conclusions:

    • IMWAT cells offer a novel and effective model for adipocyte research, derived from preadipocytes.
    • This model provides an alternative to primary adipocytes for studying adipocyte function and gene regulation.
    • The IMWAT system facilitates investigation into the role of specific factors, like RIP140, in adipocyte biology.