Bactericidal effect of TiO2 on the selected Vibrio parahaemolyticus and optimization using response surface...

Area of Science:

• Photocatalysis
• Environmental Microbiology
• Food Safety

Background:

• Vibrio parahaemolyticus is a significant pathogen affecting human and aquaculture health.
• Developing effective sterilization methods for foodborne pathogens is crucial for public health.

Purpose of the Study:

• To investigate the bactericidal efficacy of titanium dioxide (TiO2) combined with UV irradiation against Vibrio parahaemolyticus.
• To optimize sterilization parameters using response surface methodology.

Main Methods:

• Utilized Box-Behnken Design, a response surface methodology, to analyze key parameters.
• Investigated the impact of TiO2 concentration, UV illumination time, temperature, and pH on bacterial cell sterilization.
• Quantified sterilization rates based on bacterial viability loss.

Main Results:

• Maximum sterilization rate achieved at 1.0 mg/ml TiO2 concentration.
• Over 85% sterilization achieved with 30 minutes of UV irradiation (1g-TiO2/L).
• More than 99% bacterial viability loss observed after 3 hours of irradiation; sterilization improved at lower pH and temperatures.

Conclusions:

• TiO2 photocatalysis under UV light presents a potent method for Vibrio parahaemolyticus inactivation.
• Optimized conditions (TiO2 concentration, UV exposure, pH, temperature) enhance bactericidal effectiveness.
• This approach offers a promising strategy for ensuring food safety and protecting aquaculture resources.