Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Viral Recombination00:57

Viral Recombination

22.3K
Cells are sometimes infected by more than one virus at once. When two viruses disassemble to expose their genomes for replication in the same cell, similar regions of their genomes can pair together and exchange sequences in a process called recombination. Alternatively, viruses with segmented genomes can swap segments in a process called reassortment.
22.3K
Overview of Transposition and Recombination02:13

Overview of Transposition and Recombination

16.3K
Transposons make up a significant part of genomes of various organisms. Therefore, it is believed that transposition played a major evolutionary role in speciation by changing genome sizes and modifying gene expression patterns. For example, in bacteria, transposition can lead to conferring antibiotic resistance. Movement of transposable elements within the genetic pool of pathogenic bacteria can aid in transfer of antibiotic-resistant genetic elements. In eukaryotes, transposons can carry out...
16.3K
DNA-only Transposons02:57

DNA-only Transposons

16.0K
DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
The donor site from where the transposon is excised is either degraded or...
16.0K
LTR Retrotransposons03:08

LTR Retrotransposons

18.1K
LTR retrotransposons are class I transposable elements with long terminal repeats flanking an internal coding region. These elements are less abundant in mammals compared to other class I transposable elements. About 8 percent of human genomic DNA comprises LTR retrotransposons. Some of the common examples of LTR retrotransposons are Ty elements in yeast and Copia elements in Drosophila.
The internal coding region of LTR retrotransposons and their mechanism of transposition closely resembles a...
18.1K
piRNA - Piwi-interacting RNAs02:57

piRNA - Piwi-interacting RNAs

6.1K
PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
6.1K
Transposons01:24

Transposons

3.2K
Transposons, or "jumping genes," are small mobile genetic elements (MGEs) that range from 700 to 40,000 base pairs in length. They are found in all organisms and can move within the same chromosome or transfer to different chromosomes. In some cases, transposons can also jump between different host DNA molecules, such as plasmids or viruses, contributing to genetic variability.Barbara McClintock first discovered these mobile genetic elements in the 1940s while studying maize genetics, and she...
3.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Swine reporter model for preclinical evaluation and characterization of gene delivery vectors.

Molecular therapy. Advances·2026
Same author

Midgestational injection of highly expanded human CD34+ cells increases lineages of human immune cells and supports thymic development in <i>RAG2-/-IL2RG-/Y</i> SCID pigs.

Frontiers in immunology·2026
Same author

Light stress regulates pigment concentrations, growth, and gene expression in hatchery-cultivated Kappaphycus alvarezii (Solieriaceae, Rhodophyta).

Journal of phycology·2026
Same author

Development of a large porcine model of osteogenesis imperfecta type I.

Bone reports·2026
Same author

ercc6 deficient zebrafish exhibit UV and metronidazole sensitivity, increased oxygen consumption, and impaired hair cell mechanoelectrical transduction which can be restored by the superoxide dismutase mimetic MnTBAP.

Human molecular genetics·2026
Same author

Ocean transport and vertical mixing connect Greenland's macroalgae to deep ocean carbon sinks.

The Science of the total environment·2025

Related Experiment Video

Updated: May 2, 2026

piggyBac Transposon System Modification of Primary Human T Cells
10:02

piggyBac Transposon System Modification of Primary Human T Cells

Published on: November 5, 2012

16.7K

Pigs taking wing with transposons and recombinases.

Karl J Clark1, Daniel F Carlson, Scott C Fahrenkrug

  • 1Department of Animal Science at the University of Minnesota, Fitch Ave, St, Paul, MN 55108, USA.

Genome Biology
|December 6, 2007
PubMed
Summary

Genetic engineering in pigs offers agricultural and medical benefits, but challenges remain. Advances in genome sequencing and reproductive technologies are paving the way for enhanced pig transgenesis applications.

More Related Videos

The Production of Pluripotent Stem Cells from Mouse Amniotic Fluid Cells Using a Transposon System
08:24

The Production of Pluripotent Stem Cells from Mouse Amniotic Fluid Cells Using a Transposon System

Published on: February 28, 2017

6.5K
Hyperactive piggyBac Transposase-mediated Germline Transformation in the Fall Armyworm, Spodoptera frugiperda
05:20

Hyperactive piggyBac Transposase-mediated Germline Transformation in the Fall Armyworm, Spodoptera frugiperda

Published on: September 23, 2021

3.9K

Related Experiment Videos

Last Updated: May 2, 2026

piggyBac Transposon System Modification of Primary Human T Cells
10:02

piggyBac Transposon System Modification of Primary Human T Cells

Published on: November 5, 2012

16.7K
The Production of Pluripotent Stem Cells from Mouse Amniotic Fluid Cells Using a Transposon System
08:24

The Production of Pluripotent Stem Cells from Mouse Amniotic Fluid Cells Using a Transposon System

Published on: February 28, 2017

6.5K
Hyperactive piggyBac Transposase-mediated Germline Transformation in the Fall Armyworm, Spodoptera frugiperda
05:20

Hyperactive piggyBac Transposase-mediated Germline Transformation in the Fall Armyworm, Spodoptera frugiperda

Published on: September 23, 2021

3.9K

Area of Science:

  • Animal Science
  • Biotechnology
  • Genetics

Background:

  • Swine production is globally significant for meat and high-value medical products.
  • Genetic engineering in pigs has potential for agricultural and medical advancements.
  • Past limitations in pig transgenesis include poor embryo survival and high costs.

Purpose of the Study:

  • To review challenges in pig transgenesis.
  • To discuss the utility of genetic tools for improving pig engineering.
  • To highlight the potential of genetically engineered pigs for medicine and research.

Main Methods:

  • Review of existing literature on pig transgenesis.
  • Discussion of genetic engineering techniques, including transposases and recombinases.
  • Analysis of advancements in pig genome sequencing and reproductive technologies.

Main Results:

  • Despite early successes, widespread application of pig genetic engineering has been limited.
  • New technologies are revitalizing efforts in swine transgenesis.
  • Genetically modified pigs offer potential for pharmaceutical production, xenotransplantation, and disease modeling.

Conclusions:

  • Overcoming current challenges is crucial for realizing the full potential of pig genetic engineering.
  • Tools like transposases and recombinases can enhance the success and sophistication of genetic modifications.
  • Pigs are increasingly valuable models for human disease and sources for medical applications.