Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jul 9, 2026

Generation of Maternal Mutants Using zpc:cas9 Knock-in Zebrafish
09:17

Generation of Maternal Mutants Using zpc:cas9 Knock-in Zebrafish

Published on: July 22, 2025

Insertional mutagenesis strategies in zebrafish.

Sridhar Sivasubbu1, Darius Balciunas, Adam Amsterdam

  • 1Institute of Genomics and Integrative Biology, Council for Scientific and Industrial Research, Mall Road, Delhi 110007, India.

Genome Biology
|December 6, 2007
PubMed
Summary

Zebrafish insertional mutagenesis advances include retroviral methods and novel transposon strategies. These approaches offer new possibilities for gene trapping and gene breaking, enhancing genetic research capabilities.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Scube2 Modulates Coronary Vessel Formation During Cardiac Growth and Regeneration in Zebrafish.

Arteriosclerosis, thrombosis, and vascular biology·2026
Same author

Recombinant ubbR promoter enables highly efficient tamoxifen-inducible Cre recombination in embryonic and adult zebrafish.

Genetics·2026
Same author

Subtle Clinical Clues for Early Diagnosis of Lethal ORAI1 Deficiency in Infants.

Indian journal of pediatrics·2026
Same author

Validation and analysis of 12,000 AI-driven CAR-T designs in the <i>Bits to Binders</i> competitions.

bioRxiv : the preprint server for biology·2026
Same author

EpicTope: predicting and validating non-disruptive epitope tagging sites.

Development (Cambridge, England)·2026
Same author

Library transgenesis in zebrafish through delayed site-specific mosaic integration for in vivo pooled screening of transgenes.

bioRxiv : the preprint server for biology·2026

Area of Science:

  • Genetics
  • Developmental Biology
  • Molecular Biology

Background:

  • Insertional mutagenesis is a powerful tool for studying gene function.
  • Zebrafish offer advantages for genetic screens due to their rapid development and optical transparency.
  • Retroviral methods have been widely used but have limitations.

Purpose of the Study:

  • To review recent developments in insertional mutagenesis in zebrafish.
  • To highlight the strengths and weaknesses of retroviral methodologies.
  • To focus on emerging transposon-based mutagenesis approaches.

Main Methods:

  • Review of existing literature on retroviral insertional mutagenesis.
  • Analysis of current research on transposon-based systems in zebrafish.

More Related Videos

In Vivo Modeling of the Morbid Human Genome using Danio rerio
12:31

In Vivo Modeling of the Morbid Human Genome using Danio rerio

Published on: August 24, 2013

Production of Haploid Zebrafish Embryos by In Vitro Fertilization
07:54

Production of Haploid Zebrafish Embryos by In Vitro Fertilization

Published on: July 14, 2014

Related Experiment Videos

Last Updated: Jul 9, 2026

Generation of Maternal Mutants Using zpc:cas9 Knock-in Zebrafish
09:17

Generation of Maternal Mutants Using zpc:cas9 Knock-in Zebrafish

Published on: July 22, 2025

In Vivo Modeling of the Morbid Human Genome using Danio rerio
12:31

In Vivo Modeling of the Morbid Human Genome using Danio rerio

Published on: August 24, 2013

Production of Haploid Zebrafish Embryos by In Vitro Fertilization
07:54

Production of Haploid Zebrafish Embryos by In Vitro Fertilization

Published on: July 14, 2014

  • Discussion of gene trapping and gene breaking strategies.

    • Main Results:

    • Retroviral methods provide established but sometimes inefficient insertional mutagenesis.
    • Transposon-based systems offer new mechanisms for targeted gene modification.
    • Conditional gene trapping and gene breaking approaches are under development.

    Conclusions:

    • Zebrafish remain a key model organism for insertional mutagenesis studies.
    • Transposon technologies are expanding the toolkit for zebrafish genetic research.
    • Future research will likely leverage these new methods for functional genomics.