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Updated: Jul 9, 2026

Advancing High-Resolution Imaging of Virus Assemblies in Liquid and Ice
Published on: July 20, 2022
Rotational and translational alignment errors in 3D reconstruction of virus structures at high resolution
Yongchang Ji1, Dan C Marinescu
1School of Electrical Engineering and Computer Science, University of Central Florida, Orlando, FL 32816, USA. yji@cs.usf.edu
Abstract:
The 3D reconstruction of virus structures at high resolution using CryoTEM data requires a very accurate rotational and translational alignment of individual views obtained experimentally. We discuss the geometrical foundations and the computational problems raised by rotational and translational alignment. We also outline the basic ideas for CTF correction.
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