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Updated: Jul 9, 2026

Physical Isolation of Endospores from Environmental Samples by Targeted Lysis of Vegetative Cells
Published on: January 21, 2016
Bacillus kribbensis sp. nov., isolated from a soil sample in Jeju, Korea
Jee-Min Lim1, Che Ok Jeon2, Jung Ro Lee2
1Functional Metabolomics Research Center, KRIBB, Daejeon 305-806, Korea.
Abstract:
A Gram-positive, endospore-forming bacterium, designated strain BT080(T), was isolated from a soil sample in Jeju, Korea. Cells of the isolate were strictly aerobic rods that were motile by means of peritrichous flagella. The strain grew optimally at 30-33 degrees C and pH 5.5-6.5. Chemotaxonomic data (major isoprenoid quinone, MK-7; DNA G+C content, 43.3 mol%; major fatty acids, anteiso-C(15 : 0), iso-C(14 : 0), iso-C(16 : 0) and iso-C(15 : 0)) supported the affiliation of the isolate to the genus Bacillus. Comparative 16S rRNA gene sequence analyses showed that strain BT080(T) formed a distinct phyletic line within the genus Bacillus. The levels of 16S rRNA gene sequence similarity with respect to related Bacillus species were below 96.4 %. On the basis of physiological, biochemical and phylogenetic properties, strain BT080(T) represents a novel species within the genus Bacillus, for which the name Bacillus kribbensis sp. nov. is proposed. The type strain is BT080(T) (=KCTC 13934(T)=DSM 17871(T)).
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