Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

PCR - Polymerase Chain Reaction01:32

PCR - Polymerase Chain Reaction

Overview

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The German National Strategy for Gene- and Cell-Based Therapies: Generating Impact by Employing a Novel Multi-Stakeholder Approach.

Human gene therapy·2026
Same author

From Idea to Data: An Agentic Architecture for the Clinical Trial Life Cycle.

Studies in health technology and informatics·2026
Same author

LLM-Assisted Clinical Data Harmonization: Combining Automated ETL Generation with Semantic Vocabulary Mapping for OMOP CDM.

Studies in health technology and informatics·2026
Same author

Integration and validation of complementary ex vivo assays for functional precision oncology.

NPJ precision oncology·2026
Same author

Interpretable Feature Extraction from Clinical Notes for Sepsis Prediction: Comparing Rule-Based, LLM, and Hybrid Approaches.

Studies in health technology and informatics·2026
Same author

OMOP Extraction of Medical Text Using LLMs: Preliminary Results.

Studies in health technology and informatics·2026

Related Experiment Video

Updated: Jul 9, 2026

Linear Amplification Mediated PCR – Localization of Genetic Elements and Characterization of Unknown Flanking DNA
11:58

Linear Amplification Mediated PCR – Localization of Genetic Elements and Characterization of Unknown Flanking DNA

Published on: June 25, 2014

High-resolution insertion-site analysis by linear amplification-mediated PCR (LAM-PCR).

Manfred Schmidt1, Kerstin Schwarzwaelder, Cynthia Bartholomae

  • 1Department of Translational Oncology, National Center for Tumor Diseases, German Cancer Research Center, Im Neuenheimer Feld 350, 69120 Heidelberg, Germany. manfred.schmidt@nct-heidelberg.de

Nature Methods
|December 1, 2007
PubMed
Summary

Linear Amplification-Mediated PCR (LAM-PCR) precisely identifies gene therapy vector integration sites in the genome. This method tracks gene-modified cell populations and potential side effects for improved gene therapy safety.

More Related Videos

Retroviral Scanning: Mapping MLV Integration Sites to Define Cell-specific Regulatory Regions
10:10

Retroviral Scanning: Mapping MLV Integration Sites to Define Cell-specific Regulatory Regions

Published on: May 28, 2017

Amplification, Next-generation Sequencing, and Genomic DNA Mapping of Retroviral Integration Sites
09:31

Amplification, Next-generation Sequencing, and Genomic DNA Mapping of Retroviral Integration Sites

Published on: March 22, 2016

Related Experiment Videos

Last Updated: Jul 9, 2026

Linear Amplification Mediated PCR – Localization of Genetic Elements and Characterization of Unknown Flanking DNA
11:58

Linear Amplification Mediated PCR – Localization of Genetic Elements and Characterization of Unknown Flanking DNA

Published on: June 25, 2014

Retroviral Scanning: Mapping MLV Integration Sites to Define Cell-specific Regulatory Regions
10:10

Retroviral Scanning: Mapping MLV Integration Sites to Define Cell-specific Regulatory Regions

Published on: May 28, 2017

Amplification, Next-generation Sequencing, and Genomic DNA Mapping of Retroviral Integration Sites
09:31

Amplification, Next-generation Sequencing, and Genomic DNA Mapping of Retroviral Integration Sites

Published on: March 22, 2016

Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • Integrating vector systems are crucial for gene therapy, particularly in correcting immunodeficiencies.
  • Vector integration sites serve as unique molecular markers for transduced cells and their progeny.
  • Understanding clonal kinetics and vector influences is vital for gene therapy development.

Purpose of the Study:

  • To detail the application of Linear Amplification-Mediated PCR (LAM-PCR) for identifying vector-adjacent genomic sequences.
  • To demonstrate LAM-PCR's utility in analyzing gene-modified hematopoietic repopulation and clonal contributions.
  • To highlight LAM-PCR's role in uncovering insertional side effects in gene therapy studies.

Main Methods:

  • Linear Amplification-Mediated PCR (LAM-PCR) was employed for detecting and sequencing unknown DNA flanking sequences at the single-cell level.
  • The method involves preamplification of vector-genome junctions, magnetic selection for non-target DNA removal, and subsequent steps on a semisolid streptavidin phase.
  • Key steps include double-strand synthesis, restriction digest, linker cassette ligation, and exponential PCR with specific primers.

Main Results:

  • LAM-PCR enables qualitative and quantitative measurements of clonal kinetics in hematopoietic regeneration after gene transfer.
  • The technology has successfully identified the clonal derivation of both non-leukemogenic and leukemogenic insertional side effects.
  • This study specifically describes the use of LAM-PCR to identify 5' long terminal repeat (LTR) retroviral vector adjacent genomic sequences.

Conclusions:

  • LAM-PCR is a robust and reliable technology for characterizing vector integration sites in gene therapy.
  • This method provides critical insights into the dynamics of gene-modified cell populations and potential genomic alterations.
  • LAM-PCR is adaptable for analyzing unknown DNA sequences adjacent to known sequences, enhancing its utility in genetic research.