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Optimization of tannase production by Aureobasidium pullulans DBS66
Debdulal Banerjee1, Bikas R Pati
1Department of Microbiology, Vidyasagar University, Midnapore-721102, West Bengal, India. debu33@yahoo.com
Abstract:
Tannase production by Aureobasidium pullulans DBS66 was optimized. The organism produced maximum tannase in the presence of 1% tannic acid after 36 h. Maximum gallic acid accumulation was observed within 36 h and tannic acid in the fermented broth was completely degraded after 42 h of growth. Glucose had a stimulatory effect on tannase synthesis at 0.1% (w/v) concentration. The organism showed maximum tannase production with (NH4)2HPO4 as nitrogen source. Shaking speed of 120 rpm and 50-ml broth volume have been found to be suitable for maximum tannase production.
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