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Updated: Jul 9, 2026
04:30
Quantifying the Modulation of Elastase Enzyme Activity Through Colorimetric Analysis
Published on: January 17, 2025
[The study of fermentation liquid's absorption spectra in elastase fermentation]
Tian-You Yang1, Jiang-Wei Luo, Ren-Ming Liu
1Provincial Key Laboratory of Ion Beam Bioengineering, Zhengzhou University, Zhengzhou 450052, China. yangtianyou2004@163.com
Guang Pu Xue Yu Guang Pu Fen Xi = Guang Pu
|December 7, 2007
Summary
UV spectroscopy effectively tracks bacterial growth and elastase production during fermentation. This method aids in monitoring organic nitrogen bases and enzyme activity, offering a new approach for real-time fermentation analysis.
Area of Science:
- Biotechnology
- Microbiology
- Biochemistry
Background:
- Enzyme production is crucial in industrial fermentation.
- Monitoring fermentation processes in real-time is challenging.
- Spectroscopic techniques offer potential for non-invasive process monitoring.
Purpose of the Study:
- To investigate the relationship between absorption spectra and bacterial growth.
- To correlate absorption spectra with elastase production during fermentation.
- To evaluate UV spectroscopy as a method for monitoring fermentation.
Main Methods:
- Fermentation of bacteria was conducted.
- Absorption spectra of the fermentation liquid were measured.
- Bacterial growth and elastase production were quantified.
Main Results:
- Absorption spectra changes correlated significantly with bacterial growth.
- Changes in spectra also showed a strong relationship with elastase production.
- UV spectroscopy demonstrated utility in detecting changes in organic nitrogen bases and enzyme levels.
Conclusions:
- UV spectroscopic technique is a valuable tool for monitoring fermentation.
- This method provides insights into bacterial growth and enzyme production.
- The study establishes a basis for on-line fermentation monitoring using UV spectroscopy.
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