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[Simultaneous determination of 5 active components in Fructus Cnidii by HPLC]
1Zhejiang Deqing No. 3 People Hospital, Deqing 313201, China.
Objective:
To develop an RP-HPLC method for simultaneous determination of 5 constituents in Fructus Cnidii.
Method:
Analysis was performed on an Alltech C18 (4.6 mm x 250 mm, 5 microm) column. The mobile phases were acetonitrile water and acetic acid with gradient elution. The flow rate was 1 mL x min(-1). The monitoring wavelength was 325 nm and 245 nm. The column temperature was 40 degrees C.
Result:
The linear response ranges were 1-20 microg x mL(-1) (r = 0.999 9) for xanthotoxin, 1-20 microg x mL(-1) (r = 0.999 9) for isopimpinellin, 11-20 microg x mL(-1) (r = 0.999 8) for bergapten, 100-1 200 microg x mL(-1) (r = 0.999 7) for imperatorin, 100-2 000 microg x mL(-1) (r = 0.999 9) for osthole. The average recoveries were all above 95%.
Conclusion:
The method is simple, sensitive and accurate with good reproducibility.