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The Importance of Correct Protein Concentration for Kinetics and Affinity Determination in Structure-function Analysis
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Multiple cysteine residues are implicated in Janus kinase 2-mediated catalysis

Naila M Mamoon1, John K Smith, Kiranam Chatti

  • 1Department of Pharmacology and Toxicology, University of Mississippi Medical Center, Jackson, Mississippi 39216-4505, USA.

Biochemistry
|December 7, 2007
PubMed
Summary

Oxidizing conditions inactivate Janus kinase 2 (JAK2) by affecting critical cysteine residues. Reducing these residues restores JAK2 activity, revealing a direct redox regulation mechanism.

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