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Robust Ligature-Induced Model of Murine Periodontitis for the Evaluation of Oral Neutrophils

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Published on: January 21, 2020

CCL28 effects on periodontal pathogens.

Heather R Watkins¹, Carol A Lapp, Philip J Hanes

¹Department of Periodontics, Medical College of Georgia, Augusta, GA 30912, USA.

Journal of Periodontology

|December 7, 2007

Summary

Chemokine (C-C motif) ligand 28 (CCL28) effectively kills periodontal pathogens Porphyromonas gingivalis and Actinobacillus actinomycetemcomitans. Reduced salivary flow may impair oral defense by decreasing CCL28 exposure to bacteria.

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Area of Science:

Immunology
Microbiology
Biochemistry

Background:

Chemokines are crucial immune signaling proteins involved in immunity and wound healing.
CCL28, a chemokine secreted by mucosal epithelial cells, exhibits antimicrobial properties.
This study investigates CCL28's antibacterial effects on oral pathogens.

Purpose of the Study:

To determine the antibacterial activity of CCL28 against Porphyromonas gingivalis and Actinobacillus actinomycetemcomitans.
To assess the efficacy of CCL28 in killing these anaerobic periodontal pathogens.
To understand the role of CCL28 in oral self-defense mechanisms.

Main Methods:

Bacterial viability assay using fluorescent dyes to distinguish live and dead bacteria.
Exposure of P. gingivalis and A. actinomycetemcomitans to varying concentrations of CCL28 (0.04–10 microM).
Confirmation of results using standard bacterial plating techniques.

Main Results:

CCL28 demonstrated significant killing activity against both P. gingivalis and A. actinomycetemcomitans.
The 50% effective concentration (EC50) was approximately 0.7 microM for P. gingivalis and 2.0 microM for A. actinomycetemcomitans.
Bacterial viability was markedly reduced after 1-hour exposure to CCL28.

Conclusions:

CCL28 possesses potent antibacterial properties against key periodontal pathogens.
Reduced salivary flow, as seen in xerostomia, may compromise oral defense by lowering CCL28 levels.
This highlights the importance of salivary CCL28 in maintaining oral health.