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Generating Whole Bacterial Genomes from Clinical Samples using a Target Enrichment Workflow
Published on: August 15, 2025
Selective isolation of bacterial DNA from human clinical specimens
Hans-Peter Horz1, Sebastian Scheer, Frank Huenger
1Division of Oral Microbiology and Immunology, RWTH Aachen University Hospital, Germany. jhorz@ukaachen.de
Abstract:
We evaluated two DNA preparation strategies (MolYsis, Molzym GmbH & Co. KG, Bremen, Germany) and Pureprove, SIRS-Lab GmbH, Jena, Germany) to selectively extract bacterial DNA from human clinical samples. By testing 16 oral samples we found that human DNA could be largely eliminated while detectable levels of bacterial DNA were obtained with all samples. Both approaches hold great potential for microbial diagnostic systems.
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