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Updated: Jul 9, 2026
06:25
Quantifying Replication Stress in Ovarian Cancer Cells Using Single-Stranded DNA Immunofluorescence
Published on: February 10, 2023
Mechanisms to control rereplication and implications for cancer
Sara S Hook1, Jie Jessie Lin, Anindya Dutta
1Department of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, Charlottesville, VA 22908, USA. hook@virginia.edu
Current Opinion in Cell Biology
|December 7, 2007
Summary
Replication control prevents cells from duplicating DNA more than once, maintaining genomic stability and preventing cancer. When these controls fail, DNA damage and cancer risk increase.
Area of Science:
- Molecular Biology
- Cell Biology
- Genetics
Background:
- Replication initiator proteins in mammalian cells are tightly regulated by inhibitors and degradation to prevent DNA rereplication.
- Failure of these regulatory pathways triggers a G2/M checkpoint to prevent cell death after uncontrolled rereplication.
Purpose of the Study:
- To explore the role of replication control pathways in maintaining genomic stability.
- To understand how deregulation of replication licensing proteins contributes to tumorigenesis.
Main Methods:
- Analysis of patient tumors.
- Studies using genetically altered mice.
- Mammalian cell culture experiments.
Main Results:
- Deregulation of replication licensing proteins leads to increased aneuploidy.
- Chromosomal fusions and DNA breaks are observed upon loss of replication control.
- Components of rereplication-activated checkpoints are crucial for DNA repair and cancer prevention.
Conclusions:
- Replication control mechanisms are vital for preventing genomic instability and tumorigenesis.
- Dysregulation of replication licensing proteins contributes to cancer development through increased DNA damage and chromosomal abnormalities.
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02:38S-Cdk Initiates DNA Replication
The cell cycle is a series of events leading to DNA duplication followed by the division of cell content to form two daughter cells. The cell cycle progresses in four stages—the cell increases in size (gap 1 or G1-phase), duplicates its DNA (synthesis or S-phase), prepares to divide (gap 2 or G2-phase), and divides (mitosis or M-phase).
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.
02:38S-Cdk Initiates DNA Replication
The cell cycle is a series of events leading to DNA duplication followed by the division of cell content to form two daughter cells. The cell cycle progresses in four stages—the cell increases in size (gap 1 or G1-phase), duplicates its DNA (synthesis or S-phase), prepares to divide (gap 2 or G2-phase), and divides (mitosis or M-phase).
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.
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Replicative cell senescence is a property of cells that allows them to divide a finite number of times throughout the organism's lifespan while preventing excessive proliferation. Replicative senescence is associated with the gradual loss of the telomere — short, repetitive DNA sequences found at the end of the chromosomes. Telomeres are bound by a group of proteins to form a protective cap on the ends of chromosomes. Embryonic stem cells express telomerase — an enzyme that adds the telomeric...