Related Experiment Video

Updated: May 5, 2026

Real-time Imaging of Myeloid Cells Dynamics in ApcMin/+ Intestinal Tumors by Spinning Disk Confocal Microscopy
05:21

Real-time Imaging of Myeloid Cells Dynamics in ApcMin/+ Intestinal Tumors by Spinning Disk Confocal Microscopy

Published on: October 6, 2014

8.9K

Krüppel-like factor 5 mediates cellular transformation during oncogenic KRAS-induced intestinal tumorigenesis

Mandayam O Nandan1, Beth B McConnell, Amr M Ghaleb

  • 1Division of Digestive Diseases, Department of Medicine, Emory University, Atlanta, Georgia, USA.

Gastroenterology
|December 7, 2007
PubMed
Abstract

Insights

Krüppel-like factor 5 (KLF5) mediates oncogenic KRAS functions in colorectal cancer. Inhibiting KLF5 reduces tumor cell proliferation and transformation, highlighting its role in intestinal tumorigenesis.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Krüppel-like factor 5 (KLF5) is a transcription factor regulating cell proliferation.
  • Activating KRAS mutations are prevalent in colorectal cancers.
  • The role of KLF5 in KRAS-driven intestinal tumorigenesis requires elucidation.

Purpose of the Study:

  • To investigate if KLF5 mediates KRAS functions during intestinal tumorigenesis.
  • To assess the impact of KLF5 inhibition on KRAS-mutated cancer cells.

Main Methods:

  • Examined KLF5 effects on intestinal epithelial cell proliferation and transformation.
  • Utilized inducible KRAS(V12G) expression in IEC-6 cells.
  • Analyzed KLF5 expression in mouse models and human colorectal cancers with KRAS mutations.

Main Results:

  • KRAS(V12G) induction increased KLF5 expression, proliferation, and anchorage-independent growth.
  • MEK inhibitors or KLF5 siRNA reduced proliferation and transformation despite KRAS(V12G).
  • Human colorectal cancers with KRAS mutations showed high KLF5 levels and proliferation.

Conclusions:

  • Elevated KLF5 protein correlates with activating KRAS mutations in intestinal tumors.
  • KLF5 inhibition significantly reduced tumor cell proliferation and transformation.
  • KLF5 is a key mediator of oncogenic KRAS transforming functions in intestinal tumorigenesis.

More Related Videos

Improved Swiss-rolling Technique for Intestinal Tissue Preparation for Immunohistochemical and Immunofluorescent Analyses07:42

Improved Swiss-rolling Technique for Intestinal Tissue Preparation for Immunohistochemical and Immunofluorescent Analyses

Published on: July 13, 2016

70.7K
Impedance-based Real-time Measurement of Cancer Cell Migration and Invasion09:23

Impedance-based Real-time Measurement of Cancer Cell Migration and Invasion

Published on: April 2, 2020

5.9K

Related Concept Videos

Mitogens and the Cell Cycle02:38

Mitogens and the Cell Cycle

Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
6.4K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.0K
Cancer-Critical Genes I: Proto-oncogenes01:33

Cancer-Critical Genes I: Proto-oncogenes

Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
9.2K
The Ras Gene02:38

The Ras Gene

The Ras-gene-encoded proteins are regulators of signaling pathways controlling cell proliferation, differentiation, or cell survival. The Ras-gene family in humans constitutes three primary members—the HRas, NRas, and KRas. These genes code for four functionally distinct yet closely related proteins—the HRas, NRas, KRas4A, and KRas4B. The involvement of mutant Ras genes in human cancer was first discovered in 1982 and is among the most common causes of human tumorigenesis.
Ras is a...
5.7K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
3.6K
Small GTPases - Ras and Rho01:24

Small GTPases - Ras and Rho

Ras and Rho are small monomeric GTPases that act downstream of receptor tyrosine kinase (RTK) and regulate various cellular processes. These GTPases switch between active and inactive states by binding to guanine nucleotides.
Three regulatory proteins control their activity:
4.4K