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Roles of calcitonin gene-related peptide in maintenance of gastric mucosal integrity and in enhancement of ulcer healing and angiogenesis.

Takashi Ohno1, Youichiro Hattori, Rie Komine

  • 1Department of Pharmacology, Kitasato University School of Medicine, Sagamihara, Kanagawa, Japan.

Gastroenterology
|December 7, 2007

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View abstract on PubMed

Summary

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  • Biomedical And Clinical Sciences
  • Oncology And Carcinogenesis
  • Predictive And Prognostic Markers
  • Roles Of Calcitonin Gene-related Peptide In Maintenance Of Gastric Mucosal Integrity And In Enhancement Of Ulcer Healing And Angiogenesis.
    • This summary is machine-generated.

    Calcitonin gene-related peptide (CGRP) protects the gastric lining from injury and promotes ulcer healing by increasing blood vessel formation. Targeting CGRP pathways may improve gastric mucosal protection and integrity.

    Area of Science:

    • Gastroenterology
    • Neuroscience
    • Molecular Biology

    Background:

    • The gastrointestinal tract possesses rich neural systems, with afferent neurons known for protective functions.
    • Calcitonin gene-related peptide (CGRP) is an endogenous neuropeptide investigated for its role in gastric mucosal protection.

    Purpose of the Study:

    • To investigate the protective effects of CGRP against ethanol-induced gastric mucosal injury.
    • To evaluate CGRP's role in enhancing the healing of acetic acid-induced ulcers using CGRP knockout mice.

    Main Methods:

    • Gastric mucosal injury was induced by ethanol perfusion and ulcers by acetic acid application.
    • Capsaicin and NaCl were used to stimulate CGRP release, with CGRP levels measured by enzyme immunoassay.
    • Experiments were conducted on wild-type (WT) and CGRP knockout (CGRP(-/-)) mice.

    Main Results:

    • Capsaicin demonstrated a dose-dependent protective effect against ethanol-induced injury, which was abolished in CGRP(-/-) mice.
    • CGRP levels increased with capsaicin or NaCl preperfusion, reducing mucosal damage in WT but not CGRP(-/-) mice.
    • Ulcer healing was significantly delayed in CGRP(-/-) mice, with reduced granulation tissue formation, neovascularization, and vascular endothelial growth factor expression.

    Conclusions:

    • CGRP exhibits preventive action against gastric mucosal injury and possesses proangiogenic activity that enhances ulcer healing.
    • The CGRP-dependent pathway represents a promising therapeutic target for regulating gastric mucosal protection and integrity.

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