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A role for CITED2, a CBP/p300 interacting protein, in colon cancer cell invasion

Longchuan Bai1, Juanita L Merchant

  • 1Department of Internal Medicine, University of Michigan, Ann Arbor, MI 48109-2200, USA.

FEBS Letters
|December 7, 2007
PubMed

Insights

The coactivator CITED2 impacts colon cancer cell invasion and growth. Its regulation by histone deacetylase (HDAC) inhibitors suggests CITED2 as a potential therapeutic target for colon cancer.

Area of Science:

  • Molecular Biology
  • Cancer Research
  • Epigenetics

Background:

  • Histone acetyltransferase CBP/p300 regulates gene expression and cell growth, crucial for developing HDAC inhibitor strategies.
  • Understanding these mechanisms is key for effective colon cancer prevention and therapy.

Purpose of the Study:

  • To investigate the role of CITED2 in colon cancer.
  • To explore CITED2 as a potential target for HDAC inhibitor-based interventions.

Main Methods:

  • Knockdown of CITED2 in colon cancer cells.
  • Gene expression profiling to analyze MMP-13 induction.
  • Treatment with butyrate (HDAC inhibitor) in RKO cells.
  • Ectopic expression of CITED2.

Main Results:

  • CITED2 knockdown increased colon cancer cell invasiveness and MMP-13 expression.
  • Butyrate upregulated CITED2 and downregulated MMP-13 in RKO cells.
  • Ectopic CITED2 expression inhibited RKO cell growth.

Conclusions:

  • CITED2 plays a significant role in regulating colon cancer invasion and cell growth.
  • CITED2 is a potential therapeutic target for HDAC inhibitor-based colon cancer treatments.

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