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Related Concept Videos

Antigens Involved in Adaptive Immunity01:26

Antigens Involved in Adaptive Immunity

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Related Experiment Video

Updated: Jul 9, 2026

Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis
09:32

Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis

Published on: October 15, 2021

Short peptide sequences mimic HLA-DM functions.

Chih-Ling Chou1, Saied Mirshahidi, Katherine Wailen Su

  • 1Department of Pharmacology and Toxicology, University of Arizona, Tucson, AZ 85715, USA.

Molecular Immunology
|December 7, 2007
PubMed
Summary

Three novel helper peptides mimic HLA-DM (DM) function by facilitating peptide exchange on MHC class II molecules. These peptides enhance antigen presentation and T cell priming, offering new insights into immune responses.

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Last Updated: Jul 9, 2026

Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis
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A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes
07:59

A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes

Published on: March 25, 2014

Area of Science:

  • Immunology
  • Molecular Biology
  • Biochemistry

Background:

  • Human Leukocyte Antigen-DM (HLA-DM) is crucial for antigen presentation by catalyzing peptide exchange on MHC class II molecules.
  • DM interaction with MHC class II induces conformational changes, disrupting peptide binding and facilitating dissociation.

Purpose of the Study:

  • To identify specific regions or molecules that mimic HLA-DM's catalytic activity in peptide exchange.
  • To investigate the mechanism by which helper peptides accelerate DM-catalyzed peptide exchange.

Main Methods:

  • Kinetic studies were performed to analyze peptide binding and dissociation from HLA-DR1.
  • Helper peptides were tested for their ability to enhance peptide binding to DR1 on B cells in vitro.
  • T cell priming was assessed in HLA-DR1 transgenic mice using helper peptides, peptide antigen, and adjuvant.

Main Results:

  • Three short helper peptides were identified that accelerate DM-catalyzed peptide exchange.
  • These peptides enhanced peptide binding to HLA-DR1, promoted peptide-DR1 complex dissociation, and maintained an empty DR1 conformation.
  • Helper peptides increased DR1-mediated peptide binding on B cells and enhanced T cell priming in vivo.

Conclusions:

  • Helper peptides effectively mimic key functions of HLA-DM in modulating MHC class II peptide loading.
  • These findings suggest helper peptides interact with critical residues on MHC class II, similar to HLA-DM.
  • Helper peptides represent a potential tool for modulating immune responses through enhanced antigen presentation.