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Published on: June 2, 2019
Cyclic tensile strain increases interactions between human epidermal keratinocytes and quantum dot nanoparticles
Jillian G Rouse1, Carla M Haslauer, Elizabeth G Loboa
1Center for Chemical Toxicology Research and Pharmacokinetics, Department of Clinical Sciences, North Carolina State University, 4700 Hillsborough Street, Raleigh, NC 27606, USA.
Summary
Applied strain increases quantum dot (QD) uptake in human epidermal keratinocytes (HEK), negatively impacting cell viability and increasing cytokine production. This suggests physiological load affects nanoparticle interactions.
Area of Science:
- Biomedical Engineering
- Nanotechnology
- Cell Biology
Background:
- Quantum dots (QDs) show promise for pharmaceutical and biomedical applications, including diagnostics and drug delivery.
- Concerns exist regarding the cytotoxicity of QD nanoparticles due to their potential use in vivo.
- Understanding cellular responses to QDs under physiological conditions is crucial for safe application.
Purpose of the Study:
- To investigate the impact of mechanical strain on QD uptake by human epidermal keratinocytes (HEK).
- To determine if applied strain influences QD nanoparticle concentration within cells.
- To assess the effects of strain-induced QD uptake on cell viability and cytokine production.
Main Methods:
- Human epidermal keratinocytes (HEK) were cultured on collagen-coated plates.
- Cells were exposed to 3 nM of quantum dots (QDs) under a 10% average cyclic strain for 4 hours.
- Evaluated cell viability, QD uptake, and cytokine production post-exposure.
Main Results:
- Applied strain significantly increased both QD uptake and cytokine production in HEK cells.
- Increased QD nanoparticle concentration within cells was observed under strained conditions.
- Strain application led to cellular irritation and a negative impact on cell viability.
Conclusions:
- Mechanical strain enhances QD nanoparticle permeability and uptake in human epidermal keratinocytes.
- Physiological load conditions can increase intracellular QD concentration, potentially leading to cytotoxicity.
- Further research is needed to mitigate adverse effects for safe biomedical applications of QDs.
