[Comparative cytogenetic analysis of cells of mouse-mink inter-species hybridoma lines]

The comparative cytogenetic analysis of the interspecific mouse-mink hybridoma cells revealed a segregation of the great number of the mink chromosomes, inter- and intraline variability according to the number of cells with the mink DNA and its quantity in the cells. No characteristics of the mink chromosomal material distribution in the hybridoma cells which secreted the immunoglobulins of the American mink or lost its secretion were found. The great changes in the karyotype of the hybrid cells were revealed by in situ hybridization with 3H-labelled total mink DNA. Numerous insertions of the regions from the mink chromosomes to the mouse chromosomes and the appearance of the chromosomes not typical of the mink and mouse parent cells were observed. The number of cells with translocations of fragments from the chromosomes to the mouse one was observed to grow in the hybridoma cell lines cultivated for a long time. Synthesis of the lambda-L-chains of the mink immunoglobulin in the cells of line 7 was absent because they lost lambda-gene.