Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Notch Signaling Pathway03:14

Notch Signaling Pathway

The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
Notch Signaling Pathway03:14

Notch Signaling Pathway

The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
Stem Cell Niche01:26

Stem Cell Niche

The stem cell niche is the dynamic microenvironment where stem cells reside. Inside these niches, the cells may remain undifferentiated, undergo high self-renewal, or become lineage-specific progenitors. Stem cells coexist with other niche cells, such as stromal cells. They also interact closely with the ECM. Cell-cell and cell-matrix communication occur via adhesion molecules or soluble factors that signal the stem cells and determine their fate. Stromal cells also provide survival signals to...
NF-kB-dependent Signaling Pathway02:26

NF-kB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
NF-κB-dependent Signaling Pathway02:26

NF-κB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
Calmodulin-dependent Signaling01:16

Calmodulin-dependent Signaling

Calmodulin (CaM) is a calcium-binding protein in eukaryotes that controls various calcium-regulated cellular processes. It has four calcium-binding sites that bind calcium to form the calcium-calmodulin ( Ca2+-CaM) complex. GPCR stimulation increases the calcium levels in the cells that bind to CaM and induces a conformational change.
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A queen odour mediates reproductive suppression in a eusocial mammal.

Nature·2026
Same author

Cryo-EM structure of the naked mole-rat ribosome reveals a stabilized split 28S rRNA.

Nature communications·2026
Same author

Deep visual proteomics uncovers nociceptor diversity and pain targets.

Nature communications·2026
Same author

Z-DNA Hunter tool for straightforward detection of Z-DNA forming regions and a case study in <i>Drosophila</i>.

NAR genomics and bioinformatics·2025
Same author

Mechanically gated currents in mouse sensory neurons lacking PIEZO2.

Biophysical journal·2025
Same author

Cluster nanoarchitecture and structural diversity of PIEZO1 at rest and during activation in intact cells.

Science advances·2025

Related Experiment Video

Updated: Jul 9, 2026

An Optimized O9-1/Hydrogel System for Studying Mechanical Signals in Neural Crest Cells
11:02

An Optimized O9-1/Hydrogel System for Studying Mechanical Signals in Neural Crest Cells

Published on: August 13, 2021

Nociceptive tuning by stem cell factor/c-Kit signaling.

Nevena Milenkovic1, Christina Frahm, Max Gassmann

  • 1Max-Delbrück-Center for Molecular Medicine, Robert-Rössle-Strasse 10, 13092 Berlin, Germany.

Neuron
|December 7, 2007
PubMed
Summary

Stem cell factor (SCF) and its receptor, c-Kit, regulate sensory neuron sensitivity. SCF/c-Kit signaling impacts thermal pain perception and neuronal responses to stimuli.

More Related Videos

Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development
09:32

Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development

Published on: June 15, 2017

Preparation of Tunable Extracellular Matrix Microenvironments to Evaluate Schwann Cell Phenotype Specification
07:50

Preparation of Tunable Extracellular Matrix Microenvironments to Evaluate Schwann Cell Phenotype Specification

Published on: June 2, 2020

Related Experiment Videos

Last Updated: Jul 9, 2026

An Optimized O9-1/Hydrogel System for Studying Mechanical Signals in Neural Crest Cells
11:02

An Optimized O9-1/Hydrogel System for Studying Mechanical Signals in Neural Crest Cells

Published on: August 13, 2021

Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development
09:32

Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development

Published on: June 15, 2017

Preparation of Tunable Extracellular Matrix Microenvironments to Evaluate Schwann Cell Phenotype Specification
07:50

Preparation of Tunable Extracellular Matrix Microenvironments to Evaluate Schwann Cell Phenotype Specification

Published on: June 2, 2020

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Cell Signaling

Background:

  • The molecular mechanisms governing sensory circuit sensitivity to environmental stimuli remain largely unknown.
  • Understanding these mechanisms is crucial for deciphering sensory perception and pain pathways.

Purpose of the Study:

  • To investigate the role of stem cell factor (SCF) and its receptor, c-Kit, in modulating sensory neuron responsiveness.
  • To elucidate the impact of SCF/c-Kit signaling on thermal and mechanical sensory perception.

Main Methods:

  • Utilized mouse models lacking SCF/c-Kit signaling.
  • Assessed thermal thresholds and neuronal firing rates in response to heat stimuli.
  • Investigated the potentiation of heat-activated currents in isolated neurons.
  • Examined the role of TRPV1 in SCF-induced hyperalgesia.
  • Evaluated mechanoreceptive neuronal sensitivity in developmental c-Kit deficient mice.

Main Results:

  • Mice lacking SCF/c-Kit signaling exhibited thermal hypoalgesia, characterized by elevated thermal thresholds and reduced firing rates in heat-sensitive nociceptors.
  • Acute SCF activation of c-Kit reduced thermal thresholds and potentiated heat-activated currents, leading to thermal hyperalgesia.
  • SCF-induced thermal hyperalgesia was dependent on the TRPV1 channel.
  • Developmental absence of c-Kit signaling resulted in hypersensitivity of specific mechanoreceptive neuronal subtypes.

Conclusions:

  • SCF/c-Kit signaling plays a critical role in tuning the responsiveness of sensory neurons to natural stimuli, particularly thermal stimuli.
  • c-Kit functions as a key regulator of sensory transduction, influencing both hypoalgesia and hyperalgesia.
  • c-Kit joins a select group of receptor tyrosine kinases that control sensory neuron properties, offering new insights into pain modulation.