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Updated: Jul 9, 2026

Sedimentation Equilibrium of a Small Oligomer-forming Membrane Protein: Effect of Histidine Protonation on Pentameric Stability
09:49

Sedimentation Equilibrium of a Small Oligomer-forming Membrane Protein: Effect of Histidine Protonation on Pentameric Stability

Published on: April 2, 2015

Study of equilibrium solubility measurement by saturation shake-flask method using hydrochlorothiazide as model

Edit Baka1, John E A Comer, Krisztina Takács-Novák

  • 1Semmelweis University, Department of Pharmaceutical Chemistry, H-1092 Budapest Hogyes, Endre u. 9, Hungary.

Journal of Pharmaceutical and Biomedical Analysis
|December 7, 2007
PubMed

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Imagine adding a small amount of sugar to a glass of water, stirring until all the sugar has dissolved, and then adding a bit more. You can repeat this process until the sugar concentration of the solution reaches its natural limit, a limit determined primarily by the relative strengths of the solute-solute, solute-solvent, and solvent-solvent attractive forces. You can be certain that you have reached this limit because, no matter how long you stir the solution, undissolved sugar remains. The...
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